/*! This file is auto-generated */ .wp-block-button__link{color:#fff;background-color:#32373c;border-radius:9999px;box-shadow:none;text-decoration:none;padding:calc(.667em + 2px) calc(1.333em + 2px);font-size:1.125em}.wp-block-file__button{background:#32373c;color:#fff;text-decoration:none} Q14-14P If pure vitamin A has λmax =5 ... [FREE SOLUTION] | 91Ó°ÊÓ

91Ó°ÊÓ

If pure vitamin A hasλmax =5 325 (ε= 50,100), what is the vitamin A concentration in a sample whose absorbance at 325 nm is A = 0.735 in a cell with a path length of 1.00 cm?

Short Answer

Expert verified

The concentration of vitamin A is1.47×10-5 M.

Step by step solution

01

Step-by-Step Solution Step 1:Molar Absorptivity 

Molar absorptivity, also known as the molar extension coefficient represented by"ε" , is used to measure a wavelength's absorption intensity or efficiency.

Unit of Molar absorptivity is"L​â¶Ä‰mol-1 cm-1".

02

Calculation of vitamin A concentration

The formula to calculate the concentration of vitamin A is as follows:

c=Aε×±ô

Here:

  • Molar absorptivity (ε)is 5.01×104 L/³¾´Ç±ôâ‹…cm
  • Absorbance (A) is 0.735
  • The sample pathlength is 1.00 cm

The known value is substituted as:

c=0.7355.01×104 â¶Ä‹L/³¾´Ç±ô׳¦³¾Ã—1.00 c³¾=1.47×10-5 M

Thus the concentration of vitamin A is1.47×10-5 M

Unlock Step-by-Step Solutions & Ace Your Exams!

  • Full Textbook Solutions

    Get detailed explanations and key concepts

  • Unlimited Al creation

    Al flashcards, explanations, exams and more...

  • Ads-free access

    To over 500 millions flashcards

  • Money-back guarantee

    We refund you if you fail your exam.

Over 30 million students worldwide already upgrade their learning with 91Ó°ÊÓ!

One App. One Place for Learning.

All the tools & learning materials you need for study success - in one app.

Get started for free

Study anywhere. Anytime. Across all devices.