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Chapter 18: Q26P (page 458)

18-26. A 2.00mL solution of apotransferrin was titrated as illustrated in Figure 18-11. It required 163L of 1.43 mM ferric nitrilotriacetate to reach the end point.

(a) Why does the slope of the absorbance versus volume graph change abruptly at the equivalence point?

(b) How many moles of Fe(III) (= ferric nitrilotriacetate) were required to reach the end point?

(c) Each apotransferrin molecule binds two ferric ions. Find the molar concentration of apotransferrin in the 2.00mL solution.

Short Answer

Expert verified

a)Seetheexplanation.b)n(Fe)=2.33.10-7molc)[apotransferrin]=5.85.10-5M

Step by step solution

01

Reason for volume graph change abruptly at the equivalence point

a) The slope of the absorbance versus volume graph changes abruptly at the equivalence point because before the equivalence point Fe binds to a protein and forms a red complex, so the absorbance increases. After the equivalence point, there are no binding sites.

02

Find moles

b) The moles of Fe (III) that were required to reach the end point is:

n(Fe)=c.V=1.43.10-3M.163.10-6Ln(Fe)=2.33.10-7mol

03

Find apotransferrin

c) The n of apotransferrin is:

napotransferrinn(Fe)=n(Fe)2=2.33.10-7mol2napotransferrin=1.17.10-7mol

The concentration of apo transferrin is:

apotransferrin=napotransferrinVapotransferrin=1.17.10-7mol2.10-3Lapotransferrin=5.85.10-5M

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Most popular questions from this chapter

When I was a boy, Uncle Wilbur let me watch as he analyzed the iron content of runoff from his banana ranch. A 25.0 - mLsample was acidified with nitric acid and treated with excess KSCN to form a red complex. (KSCN itself iscolorless.) The solution was diluted to 100.0mLand put in a variable-pathlength cell. For comparison, a 10.0 - mLreference sample of6.80脳10-4MFe3+was treated with HNO3andand diluted to. The reference was placed in a cell with a 1.00 - mL light path. The runoff sample exhibited the same absorbance as the reference when the path length of the runoff cell was 2.48cm. What was the concentration of iron in Uncle Wilbur鈥檚 runoff?

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