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Chapter 18: Q7TY (page 444)

In a different titration, the absorbance after adding 75 mL of ferric nitrilo-triacetate to 1.500 mL of Apo transferrin was 0.222. Find the corrected absorbance.

Short Answer

Expert verified

The corrected absorbance is鈮�0.293

Step by step solution

01

Find corrected absorbance:

Correctedabsorbance=TotalvolumeInitialvolume(Observedabsorbance)

To find total volume,

Totalvolumeofsolution=VolumeofEDTA+Volumeofdrinkingwater

Given,

VolumeofEDTA=8.94mL

volume of drinking water = 5.00mL

Substitute in above equation,

Total volume of solution = 8.94mL+5.00mL

Totalvolumeofsolution=13.94mL

Correctedabsorbance=TotalvolumeInitialvolume(Observedabsorbance)

Consider,

Initial volume =5.00mL

Total volume=13.94mL

Observed absorbance =0.105

Substitute in above equation,

Correctedabsorbance=13.94mL5.00mL0.105

Corrected absorbance鈮�0.293

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Most popular questions from this chapter

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Streptavidin is a protein isolated from the bacterium Streptomyces avidinii that binds biotin with a formation constant of ~1014M-1. The biotin-streptavidin complex is widely used in biotechnology because the noncovalent complex is stable in the presence of detergents, protein denaturants, and organic solvents, and at extremes of pH and temperature.

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