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Chapter 19: Q66 (page 762)

Calculate the isoelectric point for each amino acid.

a. cysteine: pKa (COOH) = 2.05; pKa(伪-NH3+)=10.25

b. methionine: pKa(COOH) = 2.28; pKa(伪-NH3+)=9.21

Short Answer

Expert verified

a. 6.15

b. 5.75

Step by step solution

01

Step 1:Isoelectric point

The isoelectric point (PI) calculates the pH where charges are balanced. The balanced charges may acquire positive or negative charges in a particular medium.

02

Formula to calculate the PI

The formula to calculate the PI is shown as:

PI=pK1+pK2+pK3.........+pKnn

03

Step 3:Explanation

a. Isoelectric point of cysteine is calculated as:

PI=pKaCOOH+pKa伪-NH3+2=2.05+10.252=6.15b.IsoelectricpointofmethionineiscalculatedasPI=pKaCOOH+pKa伪-NH3+2=2.28+9.212=5.75

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Most popular questions from this chapter

Threonine is a naturally occurring amino acid that has two stereogenic centers.

a. Draw the four possible stereoisomers using wedges and dashes.

b. The naturally occurring amino acid has the 2S,3R configuration at its two stereogenic centers. Which structure does this correspond to?

Can octane and octan-1-ol be separated using an aqueous extraction procedure? Explain why or why not.

Question: Rank the labeled protons (Ha-Hb)in mandelic acid, a naturally occurring carboxylic acid in plumsand peaches, in order of increasing acidity. Explain in detail why you chose this order.

Question: Give the IUPAC name for each compound.

a.

b.

c.

d.

Phthalic acid and isophthalic acid have protons on two carboxy groups that can be removed with base.

(a) Explain why the pKafor loss of the first proton (pKa1) is lower for phthalic acid than isophthalic acid.

(b) Explain why the pKafor loss of the second proton ( pKa2) is higher for phthalic acid than isophthalic acid.
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