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Chapter 2: Q18 (page 40)

Question: (a) Would phosphoric acid or succinic acid be a better buffer at pH 5 ?

(b) Would ammonia or piperidine be a better buffer at pH 9 ?

(c) Would HEPES or Tris be a better buffer atpH7.5 ?

Short Answer

Expert verified

Answer

  1. Succinic acid would be a better buffer at pH 5.
  2. Ammonia would be a better buffer at pH 9.

3.HEPES would be a better buffer at pH7.5 .

Step by step solution

01

Definition of Concept

Acids: Any hydrogen-containing substance that can donate a proton (hydrogen ion) to another substance is referred to as an acid.

02

Explain which acid be a better buffer at pH 5

(a) Succinic acid has pKa of 4.3 and 5.6 , whereas phosphoric acid has pK pa of 2.14 .

Therefore, succinic acid would be a better buffer at pH 5.

03

Explain which acid be a better buffer at pH 9

(b) Ammonia have closer to pH 9 which is pH 11 while piperidine has a pH of .

Therefore, ammonia would be a better buffer at pH 9.

04

Explain which would be a better buffer at pH7.5 from HEPES or Tris

(c) HEPES has a useful pH range of 1.5 to 3.5 or 6.8 to 8.2 because it is zwitterionic and maintains physiological pH, whereas tris has a pH range of 7.1 to 9.1 but only at room temperature.

Therefore, HEPES would be a better buffer at pH7.5 .

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