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Chapter 2: Q11P (page 40)
Question: Indicate the ionic species of ammonia that predominates at pH4, 8 , and 11 .
Answer
The ionic species of ammonia are NH+4 , NH+4 and NH+3.
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You have a 5mLsample of a protein in 0.5M NaCl. You place the protein/salt sample inside dialysis tubing (see Fig. 2-14) and place the bag in a large beaker of distilled water. If your goal is to remove as much NaCl from the sample as possible, which would be more effective:
(1) placing the dialysis bag in 4L of distilled water for 12h or
(2) placing the bag in 1L of distilled water for 6 h and then in another 1L of fresh distilled water for another 6h?
Calculate the of a solution containing
(a) of ,
(b) of glycine and of , and
(c) of acetic acid and of sodium acetate (formula weight ).
Use Coulomb鈥檚 law (Equation 2-1) to explain why a salt crystal such as NaCl remains intact in benzene (C6H6) but dissociates into ions in water.
Question: Explain why water forms nearly spherical droplets on the surface of a freshly waxed car. Why doesn鈥檛 water bead on a clean windshield?
An E. coli cell contains about 2.6 x 108ions, which constitute about 1% of the mass of the cell, and each ion has an average molecular mass of40g.
(a) What is the approximate mass of the cell?
(b) The same cell contains about 2 x 108 carbohydrate molecules, which have an average molecular mass of 150g. mol-1. Approximately what percentage of the cell's mass is carbohydrate?
(c) The molecular mass of E. coli DNA is about 5.6 x 109g . mol-1, and it accounts for about 6%of the cell's mass. How many DNA molecules does the cell contain?
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