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Chapter 2: Q11P (page 40)
Question: Indicate the ionic species of ammonia that predominates at pH4, 8 , and 11 .
Answer
The ionic species of ammonia are NH+4 , NH+4 and NH+3.
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You need a buffer at for use in purifying a protein at . You have chosen Tris, . You carefully make up Tris buffer, at data-custom-editor="chemistry" , and store it in the cold to equilibrate it to the temperature of the purification. When you measure the of the temperature-equilibrated buffer it has increased to . What is the explanation for this increase? How can you avoid this problem?
Calculate the of a solution containing
(a) of ,
(b) of glycine and of , and
(c) of acetic acid and of sodium acetate (formula weight ).
You have a 5mLsample of a protein in 0.5M NaCl. You place the protein/salt sample inside dialysis tubing (see Fig. 2-14) and place the bag in a large beaker of distilled water. If your goal is to remove as much NaCl from the sample as possible, which would be more effective:
(1) placing the dialysis bag in 4L of distilled water for 12h or
(2) placing the bag in 1L of distilled water for 6 h and then in another 1L of fresh distilled water for another 6h?
Which of the functional groups listed in Table 1-2 can function as hydrogen bond donors? As hydrogen bond acceptors?
Be able to sketch a titration curve and label its parts for a monoprotic and a polyprotic acid.
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