91影视

Summarize what is known about the size and gene content of the human genome.

The recognition sequence for the restriction enzyme Taq1 is T鈫揅GA. Indicate the products of the reaction of Taq1 with the DNA sequence shown.

$$\begin{gathered} \mathbf{5}\mathbf{\text{'}}\mathbf{鈥�}\mathbf{ACGTCGAATC}\mathbf{鈥�}\mathbf{3}\mathbf{\text{'}} \\ \mathbf{3}\mathbf{\text{'}}\mathbf{鈥�}\mathbf{TGCAGCTTAG}\mathbf{鈥�}\mathbf{5}\mathbf{\text{'}} \end{gathered}$$

Explain how evolution results from mutations in DNA.

Question: Summarize the steps required to amplify a given segment of DNA in vivo and in vitro.

Using the data in Table 3-2, identify restriction enzymes that (a) produce blunt ends; (b) recognize and cleave the same sequence (called isoschizomers); (c) produce identical sticky ends.

The 13-Mb genome of the green alga Ostreococcus tauri contains $\mathbf{8000}\mathbf{}\mathbf{genes}$. Compare the gene density in this eukaryote to that of E. coli ($\mathbf{4300}\mathbf{}\mathbf{genes}$) and that of A. thaliana ($\mathbf{25000}\mathbf{}\mathbf{genes}$).

Question: Compare the properties of cloning vectors such as pUC18, bacteriophage 位, and BACs

Question: Describe the activities of the enzymes required to construct a recombinant DNA molecule.

Describe how to select recombinant clones if a foreign DNA is inserted into the polylinker site of pUC18 and then introduced into E. coli cells.

Refer to the diagram of pUC18 (Fig. 3-24) to determine which restriction enzymes you could use to insert a gene that would interfere with production of 尾-galactosidase by the host cell.