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Chapter 0: Q4-9 TY (page 1)

What mass of protein gives a corrected absorbance of 0.250?

Short Answer

Expert verified

The mass of protein giving a corrected absorbance of 0.250is15.0渭驳.

Step by step solution

01

Concept Used

Linear Calibration Curve:

The equation of the linear calibration line is:

y卤sy=m卤umx+b=ub

where,

yis the corrected absorbance.

Corrected Absorbance = Observed Absorbance - Blank Absorbance

02

Calculation of Mass of Protein

The equation of the linear calibration line is:

absorbance=mx渭驳ofprotein+by=0.00163渭驳ofprotein+0.0047

The numbers in the subscript denote insignificant figures.

The given corrected absorbance y=0.250

The mass of protein is:

=correctedabsorbance-0.00470.01630

Mass of Protein

=0.250-0.00470.01630=15.05=15.0渭驳roundedoff

Therefore, the mass of protein resulting in a corrected absorbance of 0.250is15.0渭驳

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