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Blind Samples: Interpreting Statistics-- The U.S. Department of Agriculture provided homogenized beef baby food samples to three labs for analysis.4Results from the labs agreed well for protein, fat, zinc, riboflavin, and palmitic acid. Results for iron were questionable: Lab A, 1.590.14(13); Lab B, 1.650.56 (8); Lab C, 2.680.78(3) mg/100 g . Uncertainty is the standard deviation, with the number of replicate analyses in parentheses. Use two separate t tests to compare results from Lab Cwith those from Lab A and Lab B at the 95 % confidence level. Comment on the sensibility of the t test results and offer your own conclusions.

Short Answer

Expert verified

According to the t test results, Lab C is substantially greater than Lab B but not significantly greater than Lab A. These results don't add up, so it's proposed that we increase the number of trials/replicates in Lab C.

Step by step solution

01

Definition of F test and t test

  • The F-test is used to compare standard deviations. When comparing one sample to another, or a sample to what we would expect to find given a certain population distribution, we want to determine if the spread or dispersion of the two sets of data is comparable.
  • A t-test is a statistical test used to compare the means of two groups that are related in some way.
02

Determine the F test for lab C and lab A, the t test for lab C and lab A

To utilize distinct t tests to compare the results from Lab C with those from Lab A and Lab B, we must first conduct an F test in each of them to determine which specific case of t test to apply.

(i) Lab C and Lab A

F test

Fcalc=s12s22=0.7820.142=31

Ftable= 3.88 (based on Table 4 - 3 degrees of freedom for: s1=2ands2=12)

Since Fcalc>Ftableat the 95 % confidence level, there is a considerable discrepancy between the variances and standard deviations of the two lab values.

(i) Lab C and Lab A

t test

Find the case 2 b since it is comparing two findings with standard deviations that are significantly different, i.e., role="math" localid="1663561377979" Fcalc>Ftable-

tcalc=x1-x2s12/n1+s22/n2=2.68-1.590.7823+0.14213=2.41

ttable=4.303(based on Table 4-4; degrees of freedom -2; 95 % confidence level)

Since tcalc<ttable at the 95 %confidence level, there is no significant difference between the means of the two lab reports.

03

Determine the F test for lab C and lab B, the t test for lab C and lab B

(ii) Lab C and Lab B

F test

Fcalc=s12s22=0.7820.562=1.94

Ftable=4.74 (based on Table 4-3; degrees of freedom for: s1=2and s2=7)

Since Fcalc<Ftable at the 95 %confidence level, there is no significant difference between the variances and standard deviations of the two lab values.

(ii) Lab C and Lab B

t test

Because it will compare two findings with standard deviations that aren't considerably different and select Case 2 a., i.e., Fcalc<Ftable

First, start by calculating the spooledas a precondition for solving tcalc-

Spoonled=s12(n1-1)+s22(n2-1)n1+n2-nt=0.782(3-1)+0.562(8-1)3+8-2=0.6157199941

04

Determine the tcalc and contrast it to ttable .

Now, calculate for tcalcand contrast it to ttable.

tcalc=x1-x2Spooledn1n2n1+n2=2.68-1.650.6157199941383+8=2.47

ttable=2.262 (Degrees of freedom are based on Table 4-4. localid="1663562198639" n1+n2-nt=3+8-2=9; 95% level of assurance)

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Most popular questions from this chapter

Explain the meaning of the quotation at the beginning of this chapter: 鈥淕et the right data. Get the data right. Keep the data right.鈥

How can you validate precision and accuracy?

Internal standard graph -- Data are shown below for chromatographic analysis of naphthalene (C10H8), using deuterated naphthalene (C10D8, in which D is the isotope 2H) as an internal standard. The two compounds emerge from the column at almost identical times and are measured by a mass spectrometer.

(a) Using a spreadsheet such asFigure 4-15, prepare a graph of Equation 5-12 showing peak area ratio(C10H8/C10D8)versus concentration ratio role="math" localid="1663559632352" ([C10H8]/C10D8) . Find the least-squares slope and intercept and their standard uncertainties. What is the theoretical value of the intercept? Is the observed value of the intercept within experimental uncertainty of the theoretical value?

(b) Find the quotientrole="math" localid="1663559638520" [C10H8]/[C10D8]for an unknown whose peak area ratio (C10H8/C10D8) is 0.652. Find the standard uncertainty for the peak area ratio.

(c) Here is why we try not to use 3-point calibration curves. For n = 3 data points, there is n - 2 = 1 degree of freedom, because 2 degrees of freedom are lost in computing the slope and intercept. Find the value of Student's for confidence and 1 degree of freedom. From the standard uncertainty in (b), compute the 95 % confidence interval for the quotient[C10H8]/[C10D8] . What is the percent relative uncertainty in the quotient[C10H8]/[C10D8]? Why do we avoid 3-point calibration curves?

Distinguish raw data, treated data, and results.

In a murder trial in the 1990 s, the defendant's blood was found at the crime scene. The prosecutor argued that blood was left by the defendant during the crime. The defense argued that police "planted" the defendant's blood from a sample collected later. Blood is normally collected in a vial containing the metal-binding compound EDTA (as an anticoagulant) at a concentration of ~4.5mMafter the vial is filled with blood. At the time of the trial, procedures to measure EDTA in blood were not well established. Even though the amount of EDTA found in the crime-scene blood was orders of magnitude below ~4.5mM

, the jury acquitted the defendant. This trial motivated the development of a new method to measure EDTA in blood.

(a) Precision and accuracy. To measure accuracy and precision of the method, blood was fortified with EDTA to known levels.

Accuracy=100meanvaluefound-knownvalueknownvaluePrecision=100standarddeviationmean=coeffcientofvariation

For each of the three spike levels in the table, find the precision and accuracy of the quality control samples.

(b) Detection and quantitation limits. Low concentrations of EDTA near the detection limit gave the following dimensionless instrument readings: 175,104,164,193,131,189,155,133,151, and 176. Ten blanks had a mean reading of 45 - 1 . The slope of the calibration curve is1.75199M-1. Estimate the signal and concentration detection limits and the lower limit of quantitation for EDTA.

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