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If the observed absorbance is 0.200 and the blank absorbance is 0.049,

what is the concentration of Fe (mg/mL) in the serum?

Short Answer

Expert verified

The concentration of Fe (mg/mL) in the serum is2.23μ²µ/mL

Step by step solution

01

Calculate concentration of Fe:

ConcentrationofFeinserum(μ²µ/mL)=MassofFeinserumVolume(mL)ofserum →(1)

Equation for Mass of Fe is

MassofFeinserum(μ²µ)=Correctedabsorbance-0.00150.0670

Formula for corrected absorbance is

Corrected absorbance = Observed absorbance - Blank absorbance

Given,

observed absorbance=0.200

the blank absorbance=0.049

Substitute in corrected absorbance,

localid="1663667659354" Correctedabsorbance=0.200-0.049

Corrected absorbance=0.151

Substitute above value in mass,

MassofFeinserum(μ²µ)=Correctedabsorbance-0.00150.0670

MassofFeinserum(μ²µ)=0.151-0.00150.0670

localid="1663667676297" MassofFeinserum(μ²µ)≈2.23μ²µ

Substitute in (1),

ConcentrationofFeinserum(μ²µ/mL)=MassofFemserumVolume(mL)ofserum

ConcentrationofFeinserum(μ²µ/mL)=2.23μ²µ1mL

ConcentrationofFeinserum(μ²µ/mL)=2.23μ²µ/mL

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