Chapter 21: Problem 13
In general, if we know the genomic DNA sequence of a gene we can reliably predict the nucleotide sequence of the RNA encoded by that gene. Is this statement also true for tRNAs in prokaryotes? What about tRNAs in eukaryotes?
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Chapter 21: Problem 13
In general, if we know the genomic DNA sequence of a gene we can reliably predict the nucleotide sequence of the RNA encoded by that gene. Is this statement also true for tRNAs in prokaryotes? What about tRNAs in eukaryotes?
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Assume that, in a rare instance, a typical eukaryotic triose phosphate isomerase gene contains the correct sequences to permit accurate transcription in a prokaryotic cell. Would the resulting RNA be properly translated to yield the intact enzyme?
In the promoter of the \(E\). coli lac operon the \(-10\) region has the sequence: \(5^{\prime}\)-TATGTT-3'. A mutation named UV5 changes this sequence to: \(5^{\prime}\)-TATAAT-3' (see Figure 21.6). Transcription from the lac UV5 promoter is no longer dependent on the CRP-cAMP complex. Why?
CRP-cAMP represses transcription of the \(c r p\) gene. Predict the location of the CRP-cAMP binding site relative to the promoter of the crp gene.
Why are mutations within an intron of a protein-coding gene sometimes detrimental?
Mature mRNA from eukaryotic cells is often purified from other components in the cell with the use of columns containing oligo (dT) cellulose. These columns contain short segments of single-stranded deoxyribose thymidylate residues, oligo(dT), attached to a cellulose matrix. Explain the rationale for use of these columns to purify mature mRNA from a mixture of components.
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