Chapter 13: Problem 8
What are the three basic stages of transcription? Describe what happens at each stage.
Short Answer
Step by step solution
Key Concepts
These are the key concepts you need to understand to accurately answer the question.
/*! This file is auto-generated */ .wp-block-button__link{color:#fff;background-color:#32373c;border-radius:9999px;box-shadow:none;text-decoration:none;padding:calc(.667em + 2px) calc(1.333em + 2px);font-size:1.125em}.wp-block-file__button{background:#32373c;color:#fff;text-decoration:none}
Learning Materials
Features
Discover
Chapter 13: Problem 8
What are the three basic stages of transcription? Describe what happens at each stage.
These are the key concepts you need to understand to accurately answer the question.
All the tools & learning materials you need for study success - in one app.
Get started for free
How are transcription and replication similar, and how are they different?
Assume that a mutation occurs in the gene that encodes each of the following RNA polymerases. Match the mutation with its possible effects by placing the correct letter or letters in the blanks below. There may be more than one effect for each mutated polymerase. $$\begin{array}{cc} \text{A mutation in the gene that codes for}& \text{Effects}\\\ \text{RNA polymerase I}& \---\\\ \text{ RNA polymerase II}& \--- \\ \text{RNA polymerase III}& \--- \end{array} $$ Possible effects a. tRNA is not synthesized. b. Some ribosomal RNA is not synthesized. c. Ribosomal RNA is not processed. d. pre-mRNA is not processed. e. Some mRNA molecules are not degraded. f. pre-mRNA is not synthesized.
Glenn Croston and his colleagues studied the relation between chromatin structure and transcription activity. In one set of experiments, they measured the level of in vitro transcription of a Drosophila gene by RNA polymerase II in the presence of DNA and various combinations of histone proteins (G. E. Croston et al. 1991. Science \(251: 643-649\) ). First, they measured the level of transcription of naked DNA, with no associated histone proteins. Then they measured the level of transcription after nucleosome octamers (without \(\mathrm{H} 1\) ) were added to the DNA. The addition of the octamers caused the level of transcription to drop by \(50 \% .\) When both nucleosome octamers and H1 proteins were added to the DNA, transcription was greatly repressed, dropping to less than \(1 \%\) of that obtained with naked DNA, as shown in the following table. GAL4-VP16 is a protein that binds to the DNA of certain eukaryotic genes. When GAL4-VP16 is added to DNA, the level of transcription by RNA polymerase II is greatly elevated. Even in the presence of the H1 protein, GAL4-VP16 stimulates high levels of transcription. Propose a mechanism by which the H1 protein represses transcription and by which GAL4-VP16 overcomes this repression. Explain how your proposed mechanism would produce the results obtained in these experiments.
Elaborate repair mechanisms that prevent permanent mutations in DNA are associated with replication (see Chapter 18), yet no similar repair process is associated with transcription. Can you think of a reason for this difference between replication and transcription? (Hint: Think about the relative effects of a permanent mutation in a DNA molecule and one in an RNA molecule.)
Draw an RNA nucleotide and a DNA nucleotide, highlighting the differences. How is the structure of RNA similar to that of DNA? How is it different?
What do you think about this solution?
We value your feedback to improve our textbook solutions.