Chapter 5: Q20P (page 128)
Draw a different phylogenetic tree based on the data in Problem 19.
Short Answer
The required phylogenetic tree is:
Problem presents data in a variety of ways.
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Chapter 5: Q20P (page 128)
Draw a different phylogenetic tree based on the data in Problem 19.
The required phylogenetic tree is:
Problem presents data in a variety of ways.
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Explain why a certain protein has an apparent molecular mass of 90kDwhen determined by gel filtration and 60kDwhen determined by SDS-PAGE in the presence or absence of 2-mercaptoethanol. Which molecular mass determination is more accurate?
What types of information can be retrieved from a protein sequence database?
Identify the first residue obtained by Edman degradation of cytochrome c from (a) Drosophila, (b) baker’s yeast, and (c) wheat germ (see Table 5-6).
Treatment of a polypeptide with 2-mercaptoethanol yields two polypeptides:
1. Ala–Val–Cys–Arg–Thr–Gly–Cys–Lys–Asn–Phe–Leu
2. Tyr–Lys–Cys–Phe–Arg–His–Thr–Lys–Cys–Ser
Treatment of the intact polypeptide with trypsin yields fragments with the following amino acid compositions:
3. (Ala, Arg, Cys2, Ser, Val)
4. (Arg, Cys2, Gly, Lys, Thr, Phe)
5. (Asn, Leu, Phe)
6. (His, Lys, Thr)
7. (Lys, Tyr)
Indicate the positions of the disulfide bonds in the intact polypeptide.
(a) In what order would the amino acids Arg, His, and Leu be eluted from a carboxymethyl column at pH6 ?
(b) In what order would Glu, Lys, and Val be eluted from a diethylaminoethyl column at pH 8?
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