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Chapter 5: Q19P (page 128)

Question: Sketch a phylogenetic tree for the family of homologous proteins

whose partial sequences are given below.

Protein A T L A D K A I S L H D S

Protein B T L G D K A V S I H E S

Protein C T L A D K A I S V H D S

Short Answer

Expert verified

It鈥檚 a phylogenetic tree.

Step by step solution

01

Explanation of Concept

A phylogenetic tree, also known as phylogeny, is a diagram that shows how various species, animals, or genes descended from a common ancestor.

02

Sequences of Protein

Except for the amino acid at position 9, the amino acid sequences of proteins and are identical. Protein B differs from Protein and in its sequence.


Therefore, it鈥檚 a phylogenetic tree.

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Most popular questions from this chapter

It has been hypothesized that early life-forms used only eight different amino acids to build small peptides. How many different 10-residue peptides could be constructed from eight amino acids?

You are using ammonium sulphate to purify protein Q (pI = 5.0) by salting out from a solution at pH 7.0. How should you adjust the pH of the mixture to maximize the amount of protein Q that precipitates?

You wish to sequence the light chain of a protease inhibitor from the Brassica nigra plant. Cleavage of the light chain by trypsin and chymotrypsin yields the following fragments. What is the sequence of the light chain?

Chymotrypsin

1. Leu鈥揌is鈥揕ys鈥揋ln鈥揂la鈥揂sn鈥揋ln鈥揝er鈥揋ly鈥揋ly鈥揋ly鈥揚ro鈥揝er

2. Gln鈥揋ln鈥揂la鈥揋ln鈥揌is鈥揕eu鈥揂rg鈥揂la鈥揅ys鈥揋ln鈥揋ln鈥揟rp

3. Arg鈥揑le鈥揚ro鈥揕ys鈥揅ys鈥揂rg鈥揕ys鈥揚he

Trypsin

4. Arg

5. Ala鈥揅ys鈥揋ln鈥揋ln鈥揟rp鈥揕eu鈥揌is鈥揕ys

6. Cys鈥揂rg

7. Gln鈥揂la鈥揂sn鈥揋ln鈥揝er鈥揋ly鈥揋ly鈥揋ly鈥揚ro鈥揝er

8. Phe鈥揋ln鈥揋ln鈥揂la鈥揋ln鈥揌is鈥揕eu鈥揂rg

9. Ile鈥揚ro鈥揕ys

10. Lys

What fractionation procedure could be used to purify protein 1 from a mixture of three proteins whose amino acid compositions are as follows?

1. 25% Ala, 20% Gly, 20% Ser, 10% Ile, 10% Val, 5% Asn, 5% Gln, 5% Pro

2. 30% Gln, 25% Glu, 20% Lys, 15% Ser, 10% Cys

3. 25% Asn, 20% Gly, 20% Asp, 20% Ser, 10% Lys, 5% Tyr

All three proteins are similar in size and pI, and there is no antibody available for protein 1.

What types of information can be retrieved from a protein sequence database?
See all solutions

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