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Question: Sketch a phylogenetic tree for the family of homologous proteins

whose partial sequences are given below.

Protein A T L A D K A I S L H D S

Protein B T L G D K A V S I H E S

Protein C T L A D K A I S V H D S

Short Answer

Expert verified

It’s a phylogenetic tree.

Step by step solution

01

Explanation of Concept

A phylogenetic tree, also known as phylogeny, is a diagram that shows how various species, animals, or genes descended from a common ancestor.

02

Sequences of Protein

Except for the amino acid at position 9, the amino acid sequences of proteins and are identical. Protein B differs from Protein and in its sequence.


Therefore, it’s a phylogenetic tree.

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Most popular questions from this chapter

You wish to determine the sequence of a short peptide. Cleavage with trypsin yields three smaller peptides with the sequences Leu–Glu, Gly–Tyr– Asn–Arg, and Gln–Ala–Phe–Val–Lys. Cleavage with chymotrypsin yields three peptides with the sequences Gln–Ala–Phe, Asn–Arg–Leu–Glu, and Val–Lys–Gly–Tyr. What is the sequence of the intact peptide?

Explain how you could use a column containing carboxymethyl groups to separate serum albumin and ribonuclease A(see Table 5-3).

What fractionation procedure could be used to purify protein 1 from a mixture of three proteins whose amino acid compositions are as follows?

1. 25% Ala, 20% Gly, 20% Ser, 10% Ile, 10% Val, 5% Asn, 5% Gln, 5% Pro

2. 30% Gln, 25% Glu, 20% Lys, 15% Ser, 10% Cys

3. 25% Asn, 20% Gly, 20% Asp, 20% Ser, 10% Lys, 5% Tyr

All three proteins are similar in size and pI, and there is no antibody available for protein 1.

Identify the first residue obtained by Edman degradation of cytochrome c from (a) Drosophila, (b) baker’s yeast, and (c) wheat germ (see Table 5-6).

Separate cleavage reactions of a polypeptide by CNBr and chymotrypsin yield fragments with the following amino acid sequences. What is the sequence of the intact polypeptide?

CNBr treatment

1. Arg–Ala–Tyr–Gly–Asn

2. Leu–Phe–Met

3. Asp–Met

Chymotrypsin

4. Met–Arg–Ala–Tyr

5. Asp–Met–Leu–Phe

6. Gly–Asn

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