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Chapter 5: Q14P (page 127)

Identify the first residue obtained by Edman degradation of cytochrome c from (a) Drosophila, (b) baker鈥檚 yeast, and (c) wheat germ (see Table 5-6).

Short Answer

Expert verified

a) The first residue obtained by Edman degradation of cytochrome from Drosophila is glycine.

b) The first residue obtained by Edman degradation of cytochrome from baker鈥檚 yeast is threonine.

c) The first residue obtained by Edman degradation of cytochrome from wheat germ is none.

Step by step solution

01

Definition of Edman degradation

The Edman degradation is a chemical reaction that eliminates N-terminal amino acids from a peptide in a sequential way in a sequence of steps.

02

Step 2: Identify the first residue from Drosophila

Glycine is the first residue produced via Edman degradation of Drosophila cytochrome .

03

Step 3: Identify the first residue baker’s yeast

The first residue recovered by Edman's breakdown of baker's yeast cytochrome is threonine.

04

Step 4: Identify the first residue wheat germ

The first residue recovered from the Edman degradation of wheat germ cytochrome is none.

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Protein X has an absorptivity of 0.4mL 路 mg-1路 cm-1 at 280nm. What is the absorbance at 280nm of a 2.0 mg 路 mL-1solution of protein X? (Assume the light path is 1cm.)

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Question: Purification tables are often used to keep track of the yield and purification of a protein. The specific activity is a ratio of the amount of the protein of interest, in this case Mb, obtained at a given step (渭mol or enzyme units) divided by the amount (mg) of total protein. The yield is the ratio of the amount of the protein of interest obtained at a given step (渭mol or enzyme units) divided by the original amount present in the crude extract, often converted to percent yield by multiplying by 100. The fold purification is the ratio of the specific activity of the purified protein to that of the crude preparation.

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