91Ó°ÊÓ

A separation process in which a certain quantity of a mixture like gas, solid, liquid, or enzymes is divided during a phase transition into several tiny fractions in which the composition differs according to a gradient.

This type of affinity allows for purifying a selected class of immunoglobulin and discarding the unwanted types. A particular antibody called IgG binds to solid-phase by immobilized biological ligands (proteins, lectins, etc.)

This is based on affinity purification in which antibodies in a sample bind to a particular antigen molecule through their specific antigen-binding domain. It purifies all antibodies that bind to the antigen without considering antibody class or isotypes.

It states that there is a linear relationship between the concentration and the absorbance of the suspension.

A = ɛcl

Where,

A = absorbance

É› = molar absorptivity

c = concentration

l = length of the light path

Hence, antibody can be used to purify proteins.