/*! This file is auto-generated */ .wp-block-button__link{color:#fff;background-color:#32373c;border-radius:9999px;box-shadow:none;text-decoration:none;padding:calc(.667em + 2px) calc(1.333em + 2px);font-size:1.125em}.wp-block-file__button{background:#32373c;color:#fff;text-decoration:none} Q5-3P Which peptide has greater absorb... [FREE SOLUTION] | 91影视

91影视

Log out

Learning Materials

Features

Discover

Chapter 5: Q5-3P (page 127)

Which peptide has greater absorbance at 280 nm?

A. Gln鈥揕eu鈥揋lu鈥揚he鈥揟hr鈥揕eu鈥揂sp鈥揋ly鈥揟yr

B. Ser鈥揤al鈥揟rp鈥揂sp鈥揚he鈥揋ly鈥揟yr鈥揟rp鈥揂la

Short Answer

Expert verified

Peptide B has greater absorbance because it has tryptophan and other aromatic residues.

Step by step solution

01

Tryptophan has greatest absorbance

Tryptophan has the greatest absorbance at 280nm because of its higher resonance energy transfer. Also, the fluorescence spectrum of aromatic residues such as tyrosine and phenylalanine resembles that of tryptophan.

02

Peptide B had greater absorbance

Peptide A has Gln鈥揕eu鈥揋lu鈥揚he鈥揟hr鈥揕eu鈥揂sp鈥揋ly鈥揟yr sequence. In this amino acid sequence, there is absence of tryptophan amino acids which has the highest absorbance. However, peptide B has Ser鈥揤al鈥揟rp鈥揂sp鈥揚he鈥揋ly鈥揟yr鈥揟rp鈥揂la sequence. In this amino acid sequence, there is presence of tryptophan amino acids which has the highest absorbance due to its greater absorptivity.

Hence, peptide B has greater absorbance.

Unlock Step-by-Step Solutions & Ace Your Exams!

  • Full Textbook Solutions

    Get detailed explanations and key concepts

  • Unlimited Al creation

    Al flashcards, explanations, exams and more...

  • Ads-free access

    To over 500 millions flashcards

  • Money-back guarantee

    We refund you if you fail your exam.

Over 30 million students worldwide already upgrade their learning with 91影视!

One App. One Place for Learning.

All the tools & learning materials you need for study success - in one app.

Get started for free

Most popular questions from this chapter

You must cleave the following peptide into smaller fragments. Which of the proteases listed in Table 5-4 would be likely to yield the most fragments? The fewest?

NMTQGRCKPVNTFVHEPLVDVQNVCFKE

(a) In what order would the amino acids Arg, His, and Leu be eluted from a carboxymethyl column at pH6 ?

(b) In what order would Glu, Lys, and Val be eluted from a diethylaminoethyl column at pH 8?

Protein X has an absorptivity of 0.4mL 路 mg-1路 cm-1 at 280nm. What is the absorbance at 280nm of a 2.0 mg 路 mL-1solution of protein X? (Assume the light path is 1cm.)

Explain how you would use salting out to prepare a more concentrated solution of a protein.

Separate cleavage reactions of a polypeptide by CNBr and chymotrypsin yield fragments with the following amino acid sequences. What is the sequence of the intact polypeptide?

CNBr treatment

1. Arg鈥揂la鈥揟yr鈥揋ly鈥揂sn

2. Leu鈥揚he鈥揗et

3. Asp鈥揗et

Chymotrypsin

4. Met鈥揂rg鈥揂la鈥揟yr

5. Asp鈥揗et鈥揕eu鈥揚he

6. Gly鈥揂sn
See all solutions

Recommended explanations on Biology Textbooks

Responding to Change

Read Explanation

Communicable Diseases

Read Explanation

Cell Cycle

Read Explanation

Plant Biology

Read Explanation

Microbiology

Read Explanation

Cellular Energetics

Read Explanation
View all explanations

What do you think about this solution?

We value your feedback to improve our textbook solutions.

Study anywhere. Anytime. Across all devices.