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Chapter 5: Q4P (page 127)

Protein X has an absorptivity of 0.4mL 路 mg-1路 cm-1 at 280nm. What is the absorbance at 280nm of a 2.0 mg 路 mL-1solution of protein X? (Assume the light path is 1cm.)

Short Answer

Expert verified

The absorbance at 280 nm of a 2.0 mg 路 mL鈥�1 solution of protein X is 0.8.

Step by step solution

01

Definition of protein

Proteins are huge molecules made up of fundamental building blocks called amino acids.

02

Find the absorbance of protein X

According to Beer-Law, Lambert's A =鈭�CL, where A is the absorbance of the solute. The absorptivity is;

The solute concentration is C, and the length of the light path is I in cm.

Use the formula:

A=鈭�CI

Substitute the values:

A = (0.4mLmg-1CM-1)(2.0mgmL-1)(1cm)

A = 0.8

Therefore, the total absorbance is 0.8.

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Most popular questions from this chapter

A pentapeptide has the sequenceNNKNN(using one-letter symbols for amino acids). Calculate the mass of this peptide, as determined by mass spectrometry, to three significant figures.

Explain how you would use salting out to prepare a more concentrated solution of a protein.

You must cleave the following peptide into smaller fragments. Which of the proteases listed in Table 5-4 would be likely to yield the most fragments? The fewest?

NMTQGRCKPVNTFVHEPLVDVQNVCFKE

You are using ammonium sulphate to purify protein Q (pI = 5.0) by salting out from a solution at pH 7.0. How should you adjust the pH of the mixture to maximize the amount of protein Q that precipitates?

Describe how a protein may be quantified by an assay or by absorbance spectroscopy.
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