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Chapter 5: Q15P (page 127)

A pentapeptide has the sequenceNNKNN(using one-letter symbols for amino acids). Calculate the mass of this peptide, as determined by mass spectrometry, to three significant figures.

Short Answer

Expert verified

The mass of this peptide, as determined by mass spectrometry, to three significant figures is 602g鈥�mol鈭�1.

Step by step solution

01

Definition of peptide

A molecule is made up of at least two amino acids. Proteins, which are also chains of amino acids, are larger than peptides.

02

Step 2: Calculate the mass of this peptide

Mass: Here are 4 N residues, so,4脳114=456

ASPARAGINE (N): 114gmol鈭�1N and K are connected by peptide bondsso an additional Oatom andH atoms are counted.

O鈥�:16驳鈥�mol鈭�1andH鈥�2驳鈥�mol鈭�1

456+128+6+2=602g鈥�mol鈭�1

Therefore, the mass is602g鈥�mol鈭�1 .

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Most popular questions from this chapter

You are trying to purify a protein that is soluble in a solution of 2 M ammonium sulfate. After centrifugation to remove other proteins that have precipitated at this high salt concentration, you recover the supernatant to assay the target protein鈥檚 activity in a cell culture system.

(a) Explain why the cells die when incubated with the supernatant.

(b) What procedure could you perform to correct the problem? (Hint: See Section 2-1D).

Describe how a protein may be quantified by an assay or by absorbance spectroscopy.

Explain how you would use salting out to prepare a more concentrated solution of a protein.

Electrospray ionization mass spectrometry (ESI-MS) of proteins involves creating positively charged ions of the protein and separating them according to their mass-to-charge ratio (m/z).

(a) What causes the different positive charges on different particles of the protein?

(b) The amino acid composition (in numbers of residues per chain) of hen egg-white lysozyme (HEWL) is as follows:

P 2 Y 3 N 14 H 1

D 7 M 2 L 8 E 2

C 8 R 11 G 12 F 3

A 12 I 6 K 6 V 6

S 10 W 6 T 7 Q 3

What is the maximum positive charge that can be present on a HEWL ion?

Separate cleavage reactions of a polypeptide by CNBr and chymotrypsin yield fragments with the following amino acid sequences. What is the sequence of the intact polypeptide?

CNBr treatment

1. Arg鈥揂la鈥揟yr鈥揋ly鈥揂sn

2. Leu鈥揚he鈥揗et

3. Asp鈥揗et

Chymotrypsin

4. Met鈥揂rg鈥揂la鈥揟yr

5. Asp鈥揗et鈥揕eu鈥揚he

6. Gly鈥揂sn
See all solutions

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