Learning Materials
Features
Discover
Chapter 5: Q2P (page 114)
What are the three parts of quality assurance? What questions are asked in each part and what actions are taken in each part?
The three parts of quality assurance are objectives, specifications and assessment.
Over 30 million students worldwide already upgrade their learning with 91影视!
All the tools & learning materials you need for study success - in one app.
Get started for free
In a murder trial in the 1990 s, the defendant's blood was found at the crime scene. The prosecutor argued that blood was left by the defendant during the crime. The defense argued that police "planted" the defendant's blood from a sample collected later. Blood is normally collected in a vial containing the metal-binding compound EDTA (as an anticoagulant) at a concentration of after the vial is filled with blood. At the time of the trial, procedures to measure EDTA in blood were not well established. Even though the amount of EDTA found in the crime-scene blood was orders of magnitude below
, the jury acquitted the defendant. This trial motivated the development of a new method to measure EDTA in blood.
(a) Precision and accuracy. To measure accuracy and precision of the method, blood was fortified with EDTA to known levels.
For each of the three spike levels in the table, find the precision and accuracy of the quality control samples.
(b) Detection and quantitation limits. Low concentrations of EDTA near the detection limit gave the following dimensionless instrument readings: 175,104,164,193,131,189,155,133,151, and 176. Ten blanks had a mean reading of 45 - 1 . The slope of the calibration curve is. Estimate the signal and concentration detection limits and the lower limit of quantitation for EDTA.
Standard addition graph. Students performed an experiment like that in Figure 5-7 in which each flask contained of serum, varying additions of standard, and a total volume of
(a) Prepare a standard addition graph and find in the serum.
(b) Find the standard deviation and confidence interval for .
What is a blank and what is its purpose? Distinguish method blank, reagent blank, and field blank.
Internal standard calibration curve. Figure 5-10 is a graph of in Reaction 5-13.
(d) From the uncertaintyof the intercept, find theconfidence interval for the intercept. Does this interval include the theoretical value of zero?
Standard addition graph. Allicin is a component in garlic with antimicrobial and possibly anticancer and antioxidant activity. It is unstable and therefore difficult to measure. An assay was developed in which the stable precursor alliin is added to freshly crushed garlic and converted to allicin by the enzyme alliinase found in garlic. Components of the garlic are extracted and measured by chromatography. The chromatogram shows standard additions reported as mg alliin added per gram of garlic. The chromatographic peak is allicin from the conversion of alliin.
(a)The standard addition procedure has a constant total volume. Measure the responses in the figure and prepare a graph to find how much alliin equivalent was in the unspiked garlic. The units of your answer will be mg alliin/g garlic. Find the confidence interval, as well.
(b) Given thatof alliin are converted toof allicin, find the allicin content of garlic (mg allicin/g garlic) including theconfidence interval.
What do you think about this solution?
We value your feedback to improve our textbook solutions.
Explanations 
Textbooks 
Flashcards 
91影视 AI 
Notes 
Study Plans 
Study Sets 
Find a degree 
Magazine