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Chapter 18: Q12 P (page 456)
The absorbance of a solution of a compound is 0.822 at a wavelength of 266nm in a 1.00-cm cell. Calculate the molar absorptivity at 266nm.
The Molar absorptivity at266nm is.
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Preparing standards for a calibration curve.
(a) How much ferrous ethylene diammonium sulfateshould be dissolved in a 500mL volumetric flask withto obtain a stock solution with?
(b) When making stock solution (a), you actually weighed out 1.627 g of reagent. What is the Fe concentration in role="math" localid="1668357579931" ?
(c) How would you prepare 500 mL of standard containing 1,2,3,4, andinfrom stock solution (b) using any Class A pipets from Table 2-4 with only 500 -mL volumetric flasks?
(d) To reduce the generation of chemical waste, describe how you could prepare 50 mL of standard containing, andinfrom stock solution (b) by serial dilution using any Class A pipets from Table 2-3 with only 50mL volumetric flasks?
What is the difference between luminescence and Chemi-luminescence?
Preparing standards for a calibration curve.
(a) How much ferrous ammonium sulfateshould be dissolved in a 500mL volumetric flask withto obtain a stock solution with?
(b) When making stock solution (a), you weighed out 3.627 g of reagent. What is the Fe concentration in?
(c) How would you prepare 250mL of standard containing containing infrom stock solution (b) using only 5- and 10-mL Class A pipets, only 250mL volumetric flasks, and only two consecutive dilutions of the stock solution? For example, to prepare a solution with , you could first dilute 15mL(=10+5mL) of stock solution up to 250 mL Then dilute 15mL of the new solution up to 250 mL again to get
Describe a different set of dilutions to produce 3 mg Fe/mL from 1 000 mg
Fe/mL
18-26. A 2.00mL solution of apotransferrin was titrated as illustrated in Figure 18-11. It required 163L of 1.43 mM ferric nitrilotriacetate to reach the end point.
(a) Why does the slope of the absorbance versus volume graph change abruptly at the equivalence point?
(b) How many moles of Fe(III) (= ferric nitrilotriacetate) were required to reach the end point?
(c) Each apotransferrin molecule binds two ferric ions. Find the molar concentration of apotransferrin in the 2.00mL solution.
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