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Suppose that the electroosmotic mobility of a solution is +1.61×10-7m2/(V×s)) How many plates are required to separate sulfate from bromide with a resolution of 2.0? Use Table 15-1 for mobilities and Equation 26-14 for resolution.

Short Answer

Expert verified

Plates that are required to separate sulfate from bromide with a resolution of 2.0 isN=2×510plates

Step by step solution

01

Plates that are required to separate sulfate from bromide with a resolution of 2.0

From Table 15-1 we will use the following:

μepSO42-=-8.27×10-8m/Vsμapp=μeo=μepμapp=16.1×10-8+-8.27×10-8aμapp=7.83×10-8m2/VsμepBr-=-8.13×10-8m2/Vsμapp=μeo=μepμapp=16.1×10-8+-8.13×10-8μapp=7.97×10-8m2/Vs

02

Step 2:Calculate the average

Nextwewillcalculatetheaverageμand∆μep

μav=7.83×10-8+7.97×10-82μav=7.90×10-8m2/Vs∆μep=8.27×10-8-8.13×10-8∆μep=0.14×10-8m2/Vs

03

Step 3:Equation 26-14 for resolution

Then we will use the Equation 26-14 for resolution:

N=4×Resolutionμav∆μep2N=4×2.07.900.142N=2×105plates

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Most popular questions from this chapter

(a) A particular solution in a particular capillary has an electroosmotic mobility of 1.3×10-8m2/(V×s)atpH2and 8.1 10-8m2/(V×s). How long will it take a neutral solute to travel 52 cm from the injector to the detector if 27 kV is applied across the 62-cm-long capillary tube at pH 2? At pH 12?(b) An analyte anion has an electrophoretic mobility of -1.6×10-8m2/(V×s)How long will it take to reach the detector at pH 2?At pH 12?

Explain how neutral molecules can be separated by micellar electrokinetic chromatography. Why is this a form of chromatography? Why are micelles called a pseudostationary phase?

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Find the total concentration of negative and positive charge (mol/L) to assess the quality of the analysis. What do you conclude about this analysis?

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