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The exchange capacity of an ion-exchange resin can be defined as the number of moles of charged sites per gram of dry resin. Describe how you would measure the exchange capacity of an anion-exchange resin by using standard NaOH, standard HCl, or any other reagent you wish.

Short Answer

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In this task we will explain how to measure the exchange capacity of the anion-exchange resin using the standard or standard .

Step by step solution

01

Steps to perform

  1. Wash column with NaOH in order to convert resins toOH- which contains resins.
  2. Wash with deionized water and elute the bond OH-with excess Cl2.
  3. Titrate OH-in elute with HCl to determine the moles of OH-.

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Most popular questions from this chapter

Consider a negatively charged protein adsorbed on an anion-exchange gel at pH 8.

(a) How will a gradient of eluent pH (from pH 8to some lower pH) be useful for eluting the protein? Assume that the ionic strength of the eluent is kept constant.

(b) How would a gradient of ionic strength (at constant pH) be useful for eluting the protein?

In ion-exclusion chromatography, ions are separated from nonelectrolytes by an ion-exchange column. Nonelectrolytes penetrate the stationary phase, whereas ions of the same charge as the resin are repelled by the fixed charges. Because co-ions have access to less of the column volume, electrolytes are eluted before nonelectrolytes. The chromatogram shows the separation of trichloroacetic acid (TCA,pKa=-0.5), dichloroacetic acid (DCA,), and monochloroacetic acid (MCA,pKa=2.86) by passage through a cation-exchange resin eluted with 0.01 M HCl. Explain why the three acids are separated and why they emerge in the order shown.

Blue Dextran 2000 was eluted during gel filtration in a volume of 36.4mL from a 40 * 2cm (length diameter) column of Sephadex G-50, which fractionates molecules in the molecular mass range 1500-30000.

(a) At what retention volume would hemoglobin (molecular mass 64000 ) be expected?

(b) Suppose that radioactiveNaCI22, which is not adsorbed on the column, is eluted in a volume of 109.8 mL. What would be the retention volume of a molecule with Kav= 0.65?

The migration time for Cl-in a capillary zone electrophoresis experiment is 17.12 min and the migration time for l-is 17.78 min. From mobilities in Table 15-1, predict the migration time of Br(The observed value is 19.6 min.)

(a) Suppose that the reservoir in Figure 26-7 contains 1.5 L of 2.0 M K2PO4. For how many hours can the reservoir provide 20mM KOH at a flow rate of 1.0 mL/min if 75% consumption ofin the reservoir is feasible?

(b) What starting and ending current would be required to produce a gradient from 5.0 mM KOH to 0.10 M KOH at 1.0 mL/min flow rate?

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