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Chapter 26: Q10P (page 747)

In ion-exclusion chromatography, ions are separated from nonelectrolytes by an ion-exchange column. Nonelectrolytes penetrate the stationary phase, whereas ions of the same charge as the resin are repelled by the fixed charges. Because co-ions have access to less of the column volume, electrolytes are eluted before nonelectrolytes. The chromatogram shows the separation of trichloroacetic acid (TCA,pKa=-0.5), dichloroacetic acid (DCA,), and monochloroacetic acid (MCA,pKa=2.86) by passage through a cation-exchange resin eluted with 0.01 M HCl. Explain why the three acids are separated and why they emerge in the order shown.

Short Answer

Expert verified

Ion-exclusion chromatography: Ion exclusion chromatography is used for the separation of low molecular weight ions and some natural substances by a combination of partition, adsorption, and ion repulsion.

Step by step solution

01

Elusion order

Elution order is always from most dissociated to least dissociated species and in this case, we can see from the diagram that TCA is the first one to be separated due to the lowestpKa

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Most popular questions from this chapter

The migration time for Cl-in a capillary zone electrophoresis experiment is 17.12 min and the migration time for l-is 17.78 min. From mobilities in Table 15-1, predict the migration time of Br(The observed value is 19.6 min.)

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(a) The suppressor in Figure 26-9 enables low parts-per-billion conductivity detection for anions such asCl-andBr-,.but very poor detection limits for anions such asCN-and borate. Explain why.

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A polystyrene resin molecular exclusion HPLC column has a diameter of 7.8 mm and a length of 30 cm. The solid portions of the gel particles occupy 20% of the volume, the pores occupy 40%, and the volume between particles occupies 40%. (a) At what volume would totally excluded molecules be expected to emerge? (b) At what volume would the smallest molecules be expected? (c) A mixture of polyethylene glycols of various molecular masses is eluted between 23 and 27 ml. What does this imply about the retention mechanism for these solutes on the column?
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