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Chapter 26: Q42P (page 749)
Measure the width of the peak at half-height in Figure 26-32 and calculate the plate number. The capillary length to detector was 40.0 cm. Find the plate height.
The plate number and plate height
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State three different methods to reduce electroosmotic flow. Why does the direction of electroosmotic flow change when a silica capillary is washed with a cationic surfactant?
26-D. Consider a capillary electrophoresis experiment conducted near pH9, at which the electroosmotic flow is stronger than the electrophoretic flow.
(a) Draw a picture of the capillary, showing the placement of the anode, cathode, injector, and detector. Show the direction of electroosmotic flow and the direction of electrophoretic flow of a cation and an anion. Show the direction of net flow.
(b) Using Table 15-1, explain why has a shorter migration time than Predict whether will have a shorter migration time than or a greater migration time than
(c) Why is the mobility of greater than that of?
(a) How many moles of analyte are present in asolution that occupies 1.0% of the length of acapillary?(b) What voltage is required to inject this many moles into a capillary in 4.0 s if the sample has 1/10 of the conductivity of background electrolyte, and the sample concentration is
Electroosmotic velocities of buffered solutions are shown for a bare silica capillary and one with aminopropyl groups (silica鈥擲iOCH2CH2CH2NH2) covalently attached to the wall. A positive sign means that flow is toward the cathode. Explain the signs and relative magnitudes of the velocities.
Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were separated by molecular exclusion chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate fractions of 0.65 mL were collected. The maximum of each peak came at the following fractions: ferritin, 22; transferrin, 32; and ferric citrate, 84. (That is, the ferritin peak came at an elution volume of 22 3 0.65 5 14.3 mL)Assuming that ferritin is eluted at the interstitial volume and that ferric citrate is eluted at , findfor transferrin.
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