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Chapter 26: Q41P (page 749)

The observed behavior of benzyl alcohol (C6H5CH2OH) in capillary electrophoresis is given here. Draw a graph showing the number of plates versus the electric field and explain what happens as the field increases.

Short Answer

Expert verified

When the field increases at low voltage the number of plates would increase with decreasing voltage.

Step by step solution

01

A graph showing the number of plates versus the electric field

02

Step 2:When the field increases.

  • At low voltage 鈥搕he number of plates would increase with decreasing voltage.
  • Above 25000V/m the capillary could be overheating which would decrease the number of plates due to band broadening.

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Most popular questions from this chapter

Norepinephrine (NE) in human urine can be assayed by ion-pair chromatography by using an octadecylsilane stationary phase and sodium octyl sulfate as the mobile-phase additive. Electrochemical detection (oxidation at 0.65 V versus Ag|AgCl) is used, with 2,3-dihydroxybenzylamine (DHBA) as internal standard.

  1. Explain the physical mechanism by which an ion-pair separation works.
  2. A urine sample containing an unknown amount of NE and a fixed, added concentration of DHBA gave a detector peak height ratio NE/DHAB = 0.298. Then small standard additions of NE were made, with the following results:

  3. Added concentration of NE ( ng/mL )

    Peak height ratio NE/DHBA

    12

    0.414

    24

    0.554

    36

    0.664

    48

    0.792

  • Use the graphical treatment in Section 5-3, find the original concentration of NE in the specimen.

Figure 26-23 shows the separation of substituted benzoates. There is a peak of unknown identity at 86.0 seconds. (a) Is the unknown a cation, neutral, or an anion? (b) Find the apparent mobility and electrophoretic mobility of the unknown peak.

Suppose that the electroosmotic mobility of a solution is +1.61脳10-7m2/(V脳s)) How many plates are required to separate sulfate from bromide with a resolution of 2.0? Use Table 15-1 for mobilities and Equation 26-14 for resolution.

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Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were separated by molecular exclusion chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate fractions of 0.65 mL were collected. The maximum of each peak came at the following fractions: ferritin, 22; transferrin, 32; and ferric citrate, 84. (That is, the ferritin peak came at an elution volume of 22 3 0.65 5 14.3 mL)Assuming that ferritin is eluted at the interstitial volume and that ferric citrate is eluted at Vm, findKavfor transferrin.
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