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An acid pk value indicates how powerful it is. The equilibrium constant is calculated using the negative logarithm. The pK value is affected by pH; the stronger the acid, the lower the pK value. The nucleotide pk value is determined by the trivalent connection between nitrogen and the nucleotide.

The trivalent form of N鈧僲ay be found in cytosine and uracil. Cytosine's N鈧僲olecule creates a single bond with C鈧俛nd a double bond with C鈧�. As a result, it may easily protonate and give up its proton to water, resulting in a positively charged molecule with a low pK value, similar to adenine. The N₃ atom in Uracil forms a single bond with hydrogen and two single bonds with the C₂ and C₄ atoms. As a result, it has a high pK value, which is similar to guanine, that is easily transferred its proton to water, results in a divalent and negatively charged molecule.