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Chapter 3: Q10CP (page 66)

Explain how restriction enzymes generate either sticky ends or blunt ends.

Short Answer

Expert verified

It will generate sticky or blunt ends by using the rDNA.

Step by step solution

01

Introduction

The long ends of the DNA fragment cleaved by the restriction enzyme are referred to as sticky or cohesive ends, whereas a simple cut is referred to as a blunt end.

02

Restriction enzymes

The noncohesive strands are formed when the restriction enzyme cleaves both stands. A blunt end is what it's called. Because there is no space to make proper joining, the ligase does not require these for the joining of another segment to create recombinant DNA.

For example,

5鈥�-CCC GGG-3鈥�

3鈥�-GGG CCC-5鈥�

It will produce,

5鈥�-T TGACA-3鈥�

3鈥�-AACTG T-5鈥�

03

Interpretation

Thus, the cleavage takes place at the same location where the phosphodiester connection between C and G fails to provide a cohesive site for the ligation.

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