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Chapter 3: Q10CP (page 66)

Explain how restriction enzymes generate either sticky ends or blunt ends.

Short Answer

Expert verified

It will generate sticky or blunt ends by using the rDNA.

Step by step solution

01

Introduction

The long ends of the DNA fragment cleaved by the restriction enzyme are referred to as sticky or cohesive ends, whereas a simple cut is referred to as a blunt end.

02

Restriction enzymes

The noncohesive strands are formed when the restriction enzyme cleaves both stands. A blunt end is what it's called. Because there is no space to make proper joining, the ligase does not require these for the joining of another segment to create recombinant DNA.

For example,

5鈥�-CCC GGG-3鈥�

3鈥�-GGG CCC-5鈥�

It will produce,

5鈥�-T TGACA-3鈥�

3鈥�-AACTG T-5鈥�

03

Interpretation

Thus, the cleavage takes place at the same location where the phosphodiester connection between C and G fails to provide a cohesive site for the ligation.

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Most popular questions from this chapter

Draw the tautomeric form of cytosine.

A diploid organism with a 45,000-kb haploid genome contains 21% G residues. Calculate the number of A, C, G, and T residues in the DNA of each cell in this organism.

Describe the possible outcome of a PCR experiment in which (a) one of the primers is inadvertently omitted from the reaction mixture and (b) one of the primers is complementary to several sites in the starting DNA sample.

Question: Identify the purines and pyrimidines commonly found in nucleic acids.

Using Fig. 3-3a as a guide, draw the complete structure of a nucleoside triphosphate before and after it becomes incorporated into a polynucleotide chain. Draw the structure that would result if the newly formed phosphodiester bond were hydrolyzed.
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