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Chapter 25: Q6CP (page 897)

What is the role of the metal ions in the polymerase active site?

Short Answer

Expert verified

Polymerases are responsible for the polymerization of nucleic acids.

Step by step solution

01

Different active sites of polymerases

It has different active sites for metal ions. Active sites that are structurally superimposable and highly conserved in sequencecontained by DNA polymerases.

02

Role of metal ions in polymerase active site

Metals ions arerequired for the enzymic action of polymerases.

Metal ions contribute to the catalytic process through their ability to draw in or donate electrons.

Thus, metal ions play an important role in the action of polymerases.

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Two approaches for correcting single-gene defects are gene therapy such as is discussed in Section3 - 5Dand theCRISPR - Cas9system. Explain why theCRISPR - Cas9approach can potentially provide more complete restoration of normal tissue function than gene therapy.

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