91Ó°ÊÓ

The resolution of a elution is a quantitative measure of how well two elution peaks can be differentiated in a chromatographic separation.

a) The resolution between two closely spaced peaks might be changed

- by changing the fraction of each solvent

- by changing the solvent

- by changing the PH

- by changing the stationary phase

- by changing the temperature

Question: (b) After optimization of an isocratic elution with several solvents, the resolution of two peaks is How might you improve the resolution without changing solvents or the kind of stationary phase?

Answer:

To improve the resolution without changing solvents or the kind of stationary phase we can use a different temperature, longer column, slower flow rate and smaller particle size.

The resolution of a elution is a quantitative measure of how well two elution peaks can be differentiated in a chromatographic separation.

To improve the resolution without changing solvents or the kind of stationary phase we can use a different temperature, longer column, slower flow rate and smaller particle size