91Ó°ÊÓ

To develop an isocratic separation for reversed-phase chromatography with one organic solvent.

And temperature as variables we need to change %B and temperature (T) using this steps

in run A$→$high %B and low T

in rub B$→$high %B and high T

in rub C $→$low %B and high T

in rub D $→$low %B and low T

Using the chromatograms, determine the conditions between runs A, B, C and D.

Hence,

To develop an isocratic separation for reversed-phase chromatography with one organic solvent and temperature as variables we need to change %B and temperature (T) using this steps

in run A$→$high %B and low T

in rub B$→$high %B and high T

in rub C$→$low %B and high T

in rub D$→$low %B and low T

Using the chromatograms, determine the conditions between runs A, B, C and D.