91Ó°ÊÓ

The criteria for an adequate isocratic chromatographic separation is K for all peaks must be in the range.$0.5-20$ If k is too small.

K for all peaks must be in the range $0.5-20$.

If k is too small.

The first peak must be distorted by the solvent front.

If k is too great, the separation takes too long.

The minimum resolution of $1.5$between the two closest peaks is desired.

ruggedness, a resolution of$2$ is desirable.

The pressure must be below$15\mathrm{MPa}\left(150bar\right)$so that it prolongs the life of the pump, valves, seals, and autosampler all peaks should be symmetric, with an asymmetry factor B/A in the range$0.9-1.5$.

Hence,

If k is too small. The first peak must be distorted by the solvent front. If k is too great, the separation takes too long.

The minimum resolution of $1.5$between the two closest peaks is desired.ruggedness, a resolution of $2$is desirable.