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Chapter 18: Q36P (page 460)

What is the difference between a fluorescence excitation spectrum and a fluorescence emission spectrum? Which one resembles an absorption spectrum?

Short Answer

Expert verified

The fluorescence excitation spectrum resembles an absorption spectrum.

Step by step solution

01

Differentiation:

The difference between a fluorescence excitation spectrum and a fluorescence emission spectrum is that in fluorescence excitation the位exis different, while the位emis constant In fluorescence emission it is the opposite

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Most popular questions from this chapter

18-7: How do transmittance, absorbance, and molar absorptivity differ? Which one is proportional to concentration?

Describe a different set of dilutions to produce 3 mg Fe/mL from 1 000 mg

Fe/mL

18-6. Box 3-1 showed a single-beam spectrophotometric ozone monitor. The emission spectrum of the mercury vapor lamp is shown in Box 18-2.

  1. What is the light source?
  2. What is the detector?
  3. What is the monochromator?
  4. What are the components of the optical pathlength, b?
  5. How does this instrument measure O3?

18-10. Color and absorption spectra. Color Plate 17 shows coalored solutions and their spectra. From Table 18-1, predict the color of each solution from the wavelength of maximum absorption. Do observed colors agree with predicted colors?

Ammonia can be determined spectrophotometrically by reaction with phenol in the presence of hypochlorite(OCl-)

A 4.37 - mgsample of protein was chemically digested to convert its nitrogen into ammonia and then diluted to 100.0mL. Thenof the solution were placed in a 50 - mLvolumetric flask and treated with 5mLof phenol solution plus 2mLof sodium hypochlorite solution. The sample was diluted to 50.0mLand the absorbance at 625nmwas measured in a 1.00 - cmcuvet after 30min. For reference, a standard solution was prepared from 0.0100g of NH4Cldissolved in 1.00Lof water. Then 10.0mLof this standard were placed in a50 - mL volumetric flask and analyzed in the same manner as the unknown. A reagent blank was prepared by using distilled water in place of unknown.

(a)Calculate the molar absorptivity of the blue product.

(b)Calculate the weight percent of nitrogen in the protein.
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