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Chapter 18: Q17P (page 457)
What is the purpose of neocuproine in the serum iron analysis?
The purpose of neocuproine in the serum iron analysis is the it reacts with Cu(I), so it cannot form complex with ferrozine. Ferrozine gives false positive result in analysis.
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18-26. A 2.00mL solution of apotransferrin was titrated as illustrated in Figure 18-11. It required 163L of 1.43 mM ferric nitrilotriacetate to reach the end point.
(a) Why does the slope of the absorbance versus volume graph change abruptly at the equivalence point?
(b) How many moles of Fe(III) (= ferric nitrilotriacetate) were required to reach the end point?
(c) Each apotransferrin molecule binds two ferric ions. Find the molar concentration of apotransferrin in the 2.00mL solution.
If the observed absorbance is 0.200 and the blank absorbance is 0.049,
what is the concentration of Fe (mg/mL) in the serum?
Preparing standards for a calibration curve.
(a) How much ferrous ethylene diammonium sulfateshould be dissolved in a 500mL volumetric flask withto obtain a stock solution with?
(b) When making stock solution (a), you actually weighed out 1.627 g of reagent. What is the Fe concentration in role="math" localid="1668357579931" ?
(c) How would you prepare 500 mL of standard containing 1,2,3,4, andinfrom stock solution (b) using any Class A pipets from Table 2-4 with only 500 -mL volumetric flasks?
(d) To reduce the generation of chemical waste, describe how you could prepare 50 mL of standard containing, andinfrom stock solution (b) by serial dilution using any Class A pipets from Table 2-3 with only 50mL volumetric flasks?
18-7: How do transmittance, absorbance, and molar absorptivity differ? Which one is proportional to concentration?
A compound with molecular mass 292.16g/molwas dissolved in a 5 - mLvolumetric flask. A1.00 - mLaliquot was withdrawn, placed in a 10 - mLvolumetric flask, and diluted to the mark. The absorbance atwas in 0.427 i acuvet. The molar absorptivity at 340nm is
(a) Calculate the concentration of compound in the cuvet.
(b) What was the concentration of compound in the 5 - mL flask?
(c) How many milligrams of compound were used to make the 5 - mLsolution?
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