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Chapter 26: Q37P (page 749)

Measure the number of plates for the electrophoretic peak in Figure 26-20. Use Equation 23-32 for asymmetric peaks to find the number of plates for the chromatographic peak.

Short Answer

Expert verified

The number for the electrophoretic peak isN=9.2脳104plates

The number for the chromatographic peak isN=4.1脳103plates

Step by step solution

01

Step 1:Measure the number for the electrophoretic peak

The number for the electrophoretic peak is calculated as

N=16tr2w2N=16脳6.08min20.08min2N=9.2脳104plates

02

Step 2:Measure the number for the chromatographic peak

The number for the chromatographic peak is calculated as

N=41.7tr/w0.12A/B+1.25N=41.76.03min/0.37min21.45脳1.25N=4.1脳103plates

Our measurements are 1/3 lower than the values from figure 26-20

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Most popular questions from this chapter

Figure 26-24 shows the effect on resolution of increasing voltage from 28 to120kV

(a) What is the expected ratio of migration times(t120kV/t28kV)in the two experiments? Measure the migration times for peak 1 and find the observed ratio.

(b) What is the expected ratio of plates (N120kv/N28kv)in the two experiments?

(c) What is the expected ratio of bandwidths(蟽120kv/蟽28kv)?

(d) What is the physical reason why increasing voltage decreases bandwidth and increases resolution?

(a) State the purpose of the separator and suppressor in suppressed ion chromatography. For cation chromatography, why is the suppressor an anion-exchange membrane?

In ion-exclusion chromatography, ions are separated from nonelectrolytes by an ion-exchange column. Nonelectrolytes penetrate the stationary phase, whereas ions of the same charge as the resin are repelled by the fixed charges. Because co-ions have access to less of the column volume, electrolytes are eluted before nonelectrolytes. The chromatogram shows the separation of trichloroacetic acid (TCA,pKa=-0.5), dichloroacetic acid (DCA,), and monochloroacetic acid (MCA,pKa=2.86) by passage through a cation-exchange resin eluted with 0.01 M HCl. Explain why the three acids are separated and why they emerge in the order shown.

What is electroosmosis?

Would molecular exclusion, affinity, or hydrophobic interaction chromatography be most appropriate for each of the following applications?

  1. Purifying and concentrating a crude mixture of an antibody.
  2. Desalting a solution containing a 30kDa protein.
  3. Finding the molecular mass distribution of polystyrene with 15kDa average mass.
  4. Separation of cytochrome c (12 400 Da) and ribonuclease A (12 600 Da). Cytochrome c has lower surface hydrophobicity than ribonuclease A.
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