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Chapter 26: Q27P (page 748)
What is electroosmosis?
Electroosmosis (electroendosmosis) is the pumping process in which charged particles tend to migrate to a less charged area.
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Electrophoretic mobility of the anionic form A2 of the weak acid phenol and its derivatives are:
(a) Explain the trend in electrophoretic mobility from phenol to 4-methylphenol to 4-ethylphenol.
(b) Predict the electrophoretic mobility of the analytes at pH 10.0. Explain why the predicted mobility differs from mA2. (c) The electroosmotic mobility is toward the cathode and greater in magnitude than the analyte electrophoretic mobilities. In what order will the peaks appear in the electropherogram at pH 10.0?
(a) Suppose that the reservoir in Figure 26-7 contains 1.5 L of 2.0 M . For how many hours can the reservoir provide 20mM KOH at a flow rate of 1.0 mL/min if 75% consumption ofin the reservoir is feasible?
(b) What starting and ending current would be required to produce a gradient from 5.0 mM KOH to 0.10 M KOH at 1.0 mL/min flow rate?
A gel filtration column has a radius, y.of 0.80 cm and a length,l, of 20.0 cm
(a).Calculate the volume,of the column, which is equal to
(b). The interstitial volumewas 18.1 mL and the total volume of mobile phase was 35.8 mL.
Find.for a solute eluted at 27.4 mL.
Injection in some homebuilt capillary electrophoresis instruments is performed by raising the sample vial to create a siphon. The pressure exerted by a column of water of heightiswhereis the density of water and gis the acceleration of gravity (). To what height would you need to raise the sample vial to create the necessary pressure to load the sample in 4.0 s? Is it possible to raise the inlet of this column to this height? How could you obtain the desired pressure?
Figure 26-24 shows the effect on resolution of increasing voltage from 28 to120kV
(a) What is the expected ratio of migration timesin the two experiments? Measure the migration times for peak 1 and find the observed ratio.
(b) What is the expected ratio of plates in the two experiments?
(c) What is the expected ratio of bandwidths?
(d) What is the physical reason why increasing voltage decreases bandwidth and increases resolution?
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