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Chapter 26: Q24P (page 748)

The following substances were separated on a gel filtration column. Estimate the molecular mass of the unknown.

Short Answer

Expert verified

The molecular mass is MM =105.5041=319227.3

Step by step solution

01

Define molecular mass:

Molecule mass is a molecular weight that is equal to the sum of all the atoms contained in a molecule.

02

Calculation for molecular mass of unknown:

First you need to make a graph in Excel or by hand in order to get the equation.

y=-0.0631x+7.416

Where y is log (MM) and x isVr(mL)

03

Calculation for the value of y:

Next calculate the value of y which is log (MM) considering thatVr=30.3mL

y=-0.0631x+7.416y=-0.0631脳30.3+7.416y=5.5041

Then we will calculate the molecular mass (MM)

logMM=5.5041MM=105.5041=319227.3

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Most popular questions from this chapter

Injection in some homebuilt capillary electrophoresis instruments is performed by raising the sample vial to create a siphon. The pressure exerted by a column of water of heightiswhereis the density of water and gis the acceleration of gravity (9.8m/s2). To what height would you need to raise the sample vial to create the necessary pressure to load the sample in 4.0 s? Is it possible to raise the inlet of this column to this height? How could you obtain the desired pressure?

Figure 26-23 shows the separation of substituted benzoates. There is a peak of unknown identity at 86.0 seconds. (a) Is the unknown a cation, neutral, or an anion? (b) Find the apparent mobility and electrophoretic mobility of the unknown peak.

The migration time for Cl-in a capillary zone electrophoresis experiment is 17.12 min and the migration time for l-is 17.78 min. From mobilities in Table 15-1, predict the migration time of Br(The observed value is 19.6 min.)

What is deionized water? What kind of impurities are not removed by deionization?

Measure the number of plates for the electrophoretic peak in Figure 26-20. Use Equation 23-32 for asymmetric peaks to find the number of plates for the chromatographic peak.
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