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Chapter 3: Problem 8

Calculate the isoelectric points of (a) arginine and (b) glutamate.

Short Answer

Expert verified
The isoelectric points (pI) of arginine and glutamate are 10.76 and 3.22 respectively.

Step by step solution

01

Isolation of Relevant pKa's - Arginine

Arginine has three pKa values: 2.18 (carboxyl group), 9.04 (amino group) and 12.48 (side chain). The side chain of arginine contains an additional amino group. At pH below 9.04, arginine carries a net positive charge, while at pH above this, it has a net charge of 0. Thus, for arginine, the relevant pKa's are 9.04 and 12.48.
02

Calculation of Isoelectric Point - Arginine

Find the average of the two pKa values using the formula: \(\frac{9.04 + 12.48}{2} = 10.76\). Therefore, the pI of arginine is 10.76.
03

Isolation of Relevant pKa's - Glutamate

Glutamate has three pKa values: 2.19 (carboxyl group), 4.25 (side chain, another carboxyl group) and 9.67 (amino group). The side chain of glutamate contains an additional carboxyl group. At pH below 4.25, glutamate carries a net positive charge, while at pH above this, it has a net charge of 0. Thus, for glutamate, the relevant pKa's are 2.19 and 4.25.
04

Calculation of Isoelectric Point - Glutamate

Find the average of the two pKa values using the formula: \(\frac{2.19 + 4.25}{2} = 3.22\). Therefore, the pI of glutamate is 3.22.

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