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Chapter 24: Q26P (page 1125)

Galactose can enter the glycolytic cycle but it must first react with ATP to form galactose-1-phosphate. Propose a mechanism for this reaction.

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01

Glycolytic cycle

It is also known as the embedded Meyerhof pathway. In this breakdown, glucose occurs to pyruvate with the synthesis of energy in the form of Adenosine Triphosphate and Nicotine Adenine Diphosphate, and a total of six molecules of ATP are produced in this reaction and it is occur by the catabolic pathway.

02

Mechanism

Conversion of galactose to galactose -1-phosphate is occur in the Leloir pathway which leads to producing glucose -1-phosphate the production is done when the phosphate group is joined and the ATP is converted to ADP by enzyme galactose kinase as shown below:

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Most popular questions from this chapter

What coenzyme is required to convert pyruvate to acetaldehyde?

How many molecules of ATP are obtained from the complete (including the fourth stage of catabolism) metabolism of one molecule of glucose?

What would be the results of the experiment in Problem 53 if the radioactive AMP were added to the incubation mixture instead of radioactive pyrophosphate?

Show the mechanism for the reaction of glycerol with ATP to form glycerol-3-phosphate.

A student is trying to determine the mechanism for a reaction that uses ATP to activate a carboxylate ion, which reacts with a thiol. If the carboxylate ion attacks the gamma- phosphorous of ATP, the reaction products are the thioester, ADP, and phosphate. However, whether it attacks the alpha-phosphorous or the beta-phosphorous of ATP cannot be determined from the reaction products because the thioester, AMP, and pyrophosphate would be the products in both reactions. The mechanisms can be distinguished by a labelling experiment in which the enzyme, the carboxylate ion, ATP, and radioactively labelled pyrophosphate are incubated, and then the ATP is isolated. If the isolated ATP is radioactive, the attack occurs on the alpha-phosphorous. If it is not radioactive, an attack occurs on the beta- phosphorous. Explain these conclusions.
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