Chapter 3: Q19CP (page 76)
Question: Explain why a cloning vector usually includes a selectable marker.
Short Answer
Answer
The cloning vector includes a selectable marker as it eliminates the non-transformants in the cloning vector.
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Chapter 3: Q19CP (page 76)
Question: Explain why a cloning vector usually includes a selectable marker.
Answer
The cloning vector includes a selectable marker as it eliminates the non-transformants in the cloning vector.
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Why is a genomic library larger than a cDNA library for a given organism?
Using a three-dimensional computer model of the DNA molecule (such as shown in Interactive Exercise 1), identify each of the following structural features: the 3鈥 and 5鈥 end of each strand, the atoms that make up the sugar-phosphate backbone, the major and minor grooves, the bases in several base pairs, and the atoms that participate in hydrogen bonding in A =T and G=C base pairs.
The recognition sequence for the restriction enzyme Taq1 is T鈫揅GA. Indicate the products of the reaction of Taq1 with the DNA sequence shown.
Hypoxanthine can also base-pair with cytosine. Draw the structure of this base pair.
Question: Explain how site-directed mutagenesis can be used to produce an altered protein in bacterial cells.
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