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Chapter 12: Q7. (page 373)

Write the Lineweaver鈥揃urk (double reciprocal) equation and describe the features of a Lineweaver鈥揃urk plot.

Short Answer

Expert verified

The double reciprocal equation of the Lineweaver Burk plot is:

1V=KMVmax1S+1Vmax

The main feature is that it provides a more precise estimation of Vmax on inhibition.

Step by step solution

01

Introduction

A Lineweaver Burk plot, also known as a double-reciprocal plot, is a graphical depiction of the Lineweaver Burk equation of enzyme kinetics.

02

Lineweaver-Burk plot

The Michaelis-Menten equation is:

V=KM+SVmaxS

On taking the reciprocal of both sides of the Michaelis-Menten equation, the Lineweaver-Burk plot (double reciprocal equation) is obtained.

1V=KMVmax1S+1Vmax

Where

V=Reaction velocity

KM=Michaelis-Menten constant

Vmax=Maximum reaction velocity

S=Substrate concentration

03

Lineweaver-Burk plot graphical diagram

Thus, it provides a more precise estimate of Vmaxand information on inhibition. By linearizing the data, it improvesaccuracy.

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Most popular questions from this chapter

For an enzyme-catalyzed reaction, the presence of 5 nM of a reversible inhibitor yields a Vmax value that is 80% of the value in the absenceof the inhibitor. The KM value is unchanged. (a) What type of inhibition is likely occurring? (b) What proportion of the enzyme molecules have bound inhibitor? (c) Calculate the inhibition constant.

Consider the nonenzymatic elementary reaction A 鈫� B. When the
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B produced per minute. (a) Calculate the rate constant for this reaction.
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Estimate KI for a competitive inhibitor when [I] = 5mM gives an apparent value of KM that is three times the KM for the uninhibited reaction.

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