Chapter 11: Q2. (page 327)
Describe how different enzymes are classified and named?
Short Answer
The different enzymes are generally classified on the basis of the nature of the chemical reactions they catalyze.
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Chapter 11: Q2. (page 327)
Describe how different enzymes are classified and named?
The different enzymes are generally classified on the basis of the nature of the chemical reactions they catalyze.
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What factors influence an enzyme’s substrate specificity?
The covalent catalytic mechanism of an enzyme depends on a single active site Cys whose pK is 8. A mutation in a nearby residue alters the microenvironment so that this pK increases to 10. Would the mutation cause the reaction rate to increase or decrease? Explain.
Draw a transition state diagram of (a) a non-enzymatic reaction and the corresponding enzyme-catalysed reaction in which (b) S binds loosely to the enzyme and (c) S binds very tightly to the enzyme. Compare ΔG‡ for each case. Why is tight binding of S not advantageous?
What feature of RNA would allow it to function as a ribozyme? Why are there no naturally occurring DNA enzymes?
On the free energy diagram shown, label the intermediate(s) and transition state(s). Is the reaction thermodynamically favorable?

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