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Chapter 21: Q24CP (page 764)

What are the substrates and products of the nitric oxide synthase reaction?

Short Answer

Expert verified

The reaction that converts arginine toNO and citrulline is catalyzed by nitric oxide synthase (NOS).

Step by step solution

01

 Endothelium-derived relaxing factor (EDRF).  

Arginine is the precursor of an endothelium-derived relaxing factor (EDRF) substance because it was synthesized by vascular endothelial cells and caused the underlying smooth muscle to relax.

02

 Enzyme-bound hydroxyarginine.  

The reaction proceeds via an enzyme-bound hydroxyarginine intermediate and requires an array of redox coenzymes. NOS is a homodimeric protein of 125-to 160-kD subunits, and each subunit contains one FMN, one FAD, one tetrahydrobiopterin, and one Fe(III)-heme. These cofactors facilitate the five-electron oxidation of arginine produce NO.

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