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In the actual experiment, the researchers used 149 sequences to build their sequence logo, which is shown below. There is a stack at each position, even if short, because the sequence logo includes more data. (a) Which three positions in this sequence logo have the most predictable bases? Name the most frequent base at each. (b) Which four positions have the least predictable bases? How can you tell?

Short Answer

Expert verified
  1. The three positions such as 0, 1, and 2 with the predictable bases are A, T, and G, respectively.

  2. The four positions with the least predictable bases are the -18,-15, 5, and 8.

Step by step solution

01

Description of DNA and its alignment

DNA is the genetic material present in every organism. The genetic material is composed of DNA bases such as adenine, guanine, thymine, and cytosine. The arrangement of these bases may vary in different organisms.

02

Explanation for part “a”

The three positions in the sequence logo are 0, 1, and 2. The predictable sequences present in these three positions are adenine, guanine, and thymine.

The three bases, adenine, thymine, and guanine are given at the high stack height, and are confirmed as highly predictable bases.

03

Explanation for part “b”

The least predictable bases are present in the 18, 15, 5, and 8.In these positions, the bases are seen in minimal stack height compared to the other positions.

Hence, it is confirmed as positions with the least predictable bases.

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Most popular questions from this chapter

In the sequence logo (bottom, left), the horizontal axis shows the primary sequence of the DNA by nucleotide position. Letters for each base are stacked on top of each other according to their relative frequency at that position among the aligned sequences, with the most common base as largest letter at the top of the stack. The height of each letter represents the relative frequency of that base at that position. (a) In the sequence alignment, count the number of each base at position-9 and order them from the most to least frequent. Compare this to the size and placement of each base -9 in the logo. (b) Do the same for position 0 and 1.

Which component is not directly involved in translation?

  1. GTP

  2. DNA

  3. tRNA

  4. ribosomes

The template strand of a gene contains the sequence 3’-TTCAGTCGT-5’. Imagine that the non-template plate sequence was transcribed instead of the template sequence. Draw the mRNA sequence and translate it using Figure 17.6. (Be sure to pay attention to the 5’ and 3’ ends.) Predict how well the protein synthesized from the non-template strands would function, if at all.

The template strand of a gene includes this sequence:

3’-TACTTGTCCGATATC-5’. It is mutated to

3’-TACTTGTCCAATATC-5’. For both wild-type and mutant sequences, draw the double-stranded DNA, the resulting mRNA, and the amino acid sequence each encodes. What is the effect of the mutation on the amino acid sequence?

What is a promoter? Is it located at the upstream or downstream end of a transcription unit?

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