/*! This file is auto-generated */ .wp-block-button__link{color:#fff;background-color:#32373c;border-radius:9999px;box-shadow:none;text-decoration:none;padding:calc(.667em + 2px) calc(1.333em + 2px);font-size:1.125em}.wp-block-file__button{background:#32373c;color:#fff;text-decoration:none} Q20.1-2CC. One strand of DNA molecule has t... [FREE SOLUTION] | 91Ó°ÊÓ

91Ó°ÊÓ

One strand of DNA molecule has the following sequence:

5’-°ä°Õ°Õ³Ò´¡°ä³Ò´¡°Õ°ä³Ò°Õ°Õ´¡°ä°ä³Ò-3’

Draw the other strand. Will PvuI (see question I) cut this molecule? If so, draw the products.

Short Answer

Expert verified

The DNA sequence of both strands would be:

5’-CTTGACGATCGTTACCG-3’3’-GAACTGCTAGCAATGGC-5’

Yes, PvuI can cut the given DNA sequence. The products of the cleavage are:

5’-CTTGACGAT-3’3’-GAACTGC-5’+ 5’-GTTACCG-3’3’-TAGCAATGGC-5’

Step by step solution

01

Structure of DNA

DNA comprises two strands running in opposite directions. One runs in 5’ to 3’ while the other runs in 3’ to 5’ direction. The two strands of DNA are complementary to each other and bonded through hydrogen bonds.

In DNA, thymine (T) base pairs with adenine (A) and cytosine (C) base pairs with guanine (G). Thus, the DNA sequence of the other strand is 3’-GAACTGCTAGCAATGGC-5'.

The sequence of the DNA molecule would be 5’-CTTGACGATCGTTACCG-3’3’-GAACTGCTAGCAATGGC-5’.

02

PvuI cuts the DNA molecule

The restriction enzyme PvuI recognizes 5’-CGATCG-3’ on both strands of DNA.This restriction site is present in the given DNA sequence. Therefore, PvuI recognizes the restriction site and cuts the DNA molecule within the restriction site.

03

Products of PvuI cleavage

PvuI recognizes its restriction site and cleaves the sugar-phosphate covalent bond between the T and C on each strand. As a result, two fragments of DNA with staggering ends are produced.

5’-CTTGACGAT-3’3’-GAACTGC-5’+5’-GTTACCG-3’3’-TAGCAATGGC-5’

Unlock Step-by-Step Solutions & Ace Your Exams!

  • Full Textbook Solutions

    Get detailed explanations and key concepts

  • Unlimited Al creation

    Al flashcards, explanations, exams and more...

  • Ads-free access

    To over 500 millions flashcards

  • Money-back guarantee

    We refund you if you fail your exam.

Over 30 million students worldwide already upgrade their learning with 91Ó°ÊÓ!

One App. One Place for Learning.

All the tools & learning materials you need for study success - in one app.

Get started for free

Most popular questions from this chapter

In a short essay (100–150 words), discuss how the genetic basis of life plays a central role in biotechnology.

Plants are more readily manipulated by genetic engineering than are animals because

(A) plant genes do not contain introns.

(B) more vectors are available for transferring recombinant

DNA into plant cells.

(C) a somatic plant cell can often give rise to a complete

plant.

(D) plant cells have larger nuclei.

Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme?

(A) AAGG

TTCC

(B) GGCC

CCGG

(C) ACCA

TGGT

(D) AAAA

TTTT

You are cloning an aardvark gene, using a bacterial plasmid as a vector. The green diagram shows the plasmid, which contains the restriction site for the enzyme used in Figure 20.5. Above the plasmid is a segment of linear aardvark DNA that was synthesized using PCR. Diagram your cloning procedure, and show what would happen to these two molecules during each step. Use one color for the aardvark DNA and its bases and another color for those of the plasmid. Label each step and all 5’ and 3’ ends.

If the template strand has two or more identical nucleotides in a row, their complementary nucleotides will be added one after the other in the same flow step. How are two or more of the same nucleotide (in a row) detected in the flowgram? (See sample on the right.) Write out the sequence of the first 25 nucleotides in the flow-gram above, starting from the left (Ignore the very short lines.)

See all solutions

Recommended explanations on Biology Textbooks

View all explanations

What do you think about this solution?

We value your feedback to improve our textbook solutions.

Study anywhere. Anytime. Across all devices.