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Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme?

(A) AAGG

TTCC

(B) GGCC

CCGG

(C) ACCA

TGGT

(D) AAAA

TTTT

Short Answer

Expert verified

(A) The option 鈥淎AGG-TTCC鈥 is false.

(B) The option 鈥淕GCC-CCGG鈥 is true.

(C) The option 鈥淎CCA-TGGT鈥 is false.

(D)The option 鈥淎AAA-TTTT鈥 is false.

Step by step solution

01

Step 1: Restriction Enzyme

Restriction enzymes are types of endonucleases that cut DNA molecules at specific sites to get the DNA fragments. They are divided into five types on the basis of the site of restriction.

They break double-stranded DNA by making breaks in both strands in the backbones. So they are important tools in recombinant DNA technology.

02

Explanation of option ‘(A)’

Restriction enzymes cleave the DNA at their recognition sequences that are called restriction sites that are generally symmetrical. The sequence has a similar nucleotide sequence when read in the same orientation.

Thus, AAGG-TTCC is not a symmetrical sequence.

Therefore, the given option is false.

03

Explanation of option ‘(B)’

When the first strand is read in 5鈥 to 3鈥 direction, it is GGCC, and when the second strand is read in 5鈥 to 3鈥 direction, it is also GGCC. So it is a symmetrical sequence.

Thus, the sequence given in the option can be recognized by restriction enzymes.

Therefore, the given option is true.

04

Explanation of option ‘(C)’

When the first strand is read in 5鈥 to 3鈥 direction, it is ACCA, and when the second strand is read in 5鈥 to 3鈥 direction, it is TGGT. The sequences in both strands are different, so it is not a symmetrical sequence.

Thus, the restriction enzyme only cuts the symmetrical sequences.

Therefore, the given statement is false.

05

Explanation of option ‘(D)’

Restriction enzyme only cuts at the restriction sites that have palindromic or symmetrical sequences.

Thus, the sequence mentioned in the option is not symmetrical.

Therefore, the given statement is false.

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Most popular questions from this chapter

If the template strand has two or more identical nucleotides in a row, their complementary nucleotides will be added one after the other in the same flow step. How are two or more of the same nucleotide (in a row) detected in the flowgram? (See sample on the right.) Write out the sequence of the first 25 nucleotides in the flow-gram above, starting from the left (Ignore the very short lines.)

One strand of DNA molecule has the following sequence:

5鈥-颁罢罢骋础颁骋础罢颁骋罢罢础颁颁骋-3鈥

Draw the other strand. Will PvuI (see question I) cut this molecule? If so, draw the products.

Compare Figure 20.7 with Figure 16.20. How does replication of DNA ends during PCR proceed without shortening the fragments each time?

You are cloning an aardvark gene, using a bacterial plasmid as a vector. The green diagram shows the plasmid, which contains the restriction site for the enzyme used in Figure 20.5. Above the plasmid is a segment of linear aardvark DNA that was synthesized using PCR. Diagram your cloning procedure, and show what would happen to these two molecules during each step. Use one color for the aardvark DNA and its bases and another color for those of the plasmid. Label each step and all 5鈥 and 3鈥 ends.

A paleontologist has recovered a bit of tissue from the 400-year old preserved skin of an extinct dodo (a bird). To compare a specific region of the DNA from a sample with DNA from living birds, which of the following would be most helpful in increasing the amount of dodo DNA available for testing?

(A) SNP analysis

(B) polymerase chain reaction (PCR)

(C) electroporation

(D) gel electrophoresis

See all solutions

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