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A paleontologist has recovered a bit of tissue from the 400-year old preserved skin of an extinct dodo (a bird). To compare a specific region of the DNA from a sample with DNA from living birds, which of the following would be most helpful in increasing the amount of dodo DNA available for testing?

(A) SNP analysis

(B) polymerase chain reaction (PCR)

(C) electroporation

(D) gel electrophoresis

Short Answer

Expert verified

(A) The option 鈥淪NP analysis鈥 is false.

(B) The option 鈥減olymerase chain reaction (PCR)鈥 is true.

(C) The option 鈥渆lectroporation鈥 is false.

(D)The option 鈥済el electrophoresis鈥 is false.

Step by step solution

01

Step 1: Dodo

Dodo is a bird that is a member of the family Raphidae that is the extinct bird of Mauritius. It is flightless, so it did not survive due to predation by human beings. It is larger than a turkey and has blue-grey plumage. It was seen in 1662 lastly. Nicobar pigeons are considered the closest relative of the dodo.

02

Explanation of option ‘(A)’

SNP(single nucleotide Polymorphism) analysis is done to find out the genes contributing to a disease or disorder on the basis of the variation in a single base pair.

Thus, SNP analysis is not used for the multiplication of DNA molecules.

Therefore, the given option is false.

03

Explanation of option ‘(B)’

The polymerase chain reaction is the technique by which many copies of the desired gene can be obtained that are used for further polymorphic studies and making genomic libraries. It can produce billions of copies of the desired DNA segment from a very minute amount of DNA sample in a few hours.

Thus, the polymerase chain reaction will be helpful in increasing the amount of DNA of Dodo.

Therefore, the given option is true.

04

Explanation of option ‘(C)’

Electroporation is the DNA transfer technique in which the access of a cell to a DNA molecule is increased by increasing the permeability of the cell membrane by using an electric field.

Thus, electroporation is the technique helping in the transfer of the DNA to a cell.

Therefore, the given statement is false.

05

Explanation of option ‘(D)’

Gel electrophoresis is the separation of DNA and other molecules by passing them through a gel in an electric field.

Thus, gel electrophoresis is not used to increase the amount of DNA.

Therefore, the given statement is false.

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