/*! This file is auto-generated */ .wp-block-button__link{color:#fff;background-color:#32373c;border-radius:9999px;box-shadow:none;text-decoration:none;padding:calc(.667em + 2px) calc(1.333em + 2px);font-size:1.125em}.wp-block-file__button{background:#32373c;color:#fff;text-decoration:none} Q20-2TYU. Which of the following tools of ... [FREE SOLUTION] | 91影视

91影视

Which of the following tools of DNA technology is incorrectly paired with its use?

(A) electrophoresis鈥攕eparation of DNA fragments

(B) DNA ligase鈥攃utting DNA, creating sticky ends of restriction

fragments

(C) DNA polymerase鈥攑olymerase chain reaction to amplify

sections of DNA

(D) reverse transcriptase鈥攑roduction of cDNA from

mRNA

Short Answer

Expert verified

(A) The option 鈥渆lectrophoresis鈥攕eparation of DNA fragments鈥 is false.

(B) The option 鈥淒NA ligase鈥攃utting DNA, creating sticky ends of restriction fragments鈥 is true.

(C) The option 鈥淒NA polymerase鈥攑olymerase chain reaction to amplify

sections of DNA鈥 is false.

(D)The option 鈥渞everse transcriptase鈥攑roduction of cDNA from mRNA鈥 is false.

Step by step solution

01

Step 1: Tools of DNA technology

DNA technology is the molecular technique that involves finding the desired DNA fragments from the genome of an organism, inserting them in a suitable vector, and forming recombinant DNA. After this, it is multiplied in a host.

The task of DNA technology is not accomplished without using some tools that are mandatory to use. These tools constitute a vector, restriction enzymes, other enzymes like DNA ligase, DNA polymerase, reverse transcriptase, a host organism, and gel electrophoresis.

02

Explanation of option ‘(A)’

Electrophoresis is a technique that involves the use of a gel matrix to separate DNA and proteins on the basis of the size of molecules and the electric charge present on them.

Thus, electrophoresis and separation of DNA fragments are correctly paired.

Therefore, the given option is false.

03

Explanation of option ‘(B)’

DNA ligase is an enzyme that catalyzes the synthesis reaction that involves the formation of a phosphodiester bond between two DNA strands and joining them. DNA molecules are cut and digested by using restriction enzymes that also create sticky ends in DNA fragments.

Thus, cutting DNA and creatingsticky ends in DNA fragments do not involve DNA ligases.

Therefore, the given option is true.

04

Explanation of option ‘(C)’

DNA polymerases are enzymes involved in genome replication and maintenance and are also used in DNA technology to amplify the desired DNA fragments. They are used for DNA cloning, polymerase chain reaction, and genome sequencing.

Thus, DNA polymerases are used to amplify a section of DNA in the polymerase chain reaction.

Therefore, the given statement is false.

05

Explanation of option ‘(D)’

Reverse transcriptase is the enzyme that catalyzes the reverse process of central dogma that is reverse transcription. It makes complementary DNA copies of an RNA molecule.

Thus, reverse transcriptase synthesizes DNA copies from mRNA that is called complementary DNA.

Therefore, the given statement is false.

Unlock Step-by-Step Solutions & Ace Your Exams!

  • Full Textbook Solutions

    Get detailed explanations and key concepts

  • Unlimited Al creation

    Al flashcards, explanations, exams and more...

  • Ads-free access

    To over 500 millions flashcards

  • Money-back guarantee

    We refund you if you fail your exam.

Over 30 million students worldwide already upgrade their learning with 91影视!

One App. One Place for Learning.

All the tools & learning materials you need for study success - in one app.

Get started for free

Most popular questions from this chapter

Ethical considerations aside, if DNA-based technologies became widely used, discuss how they might change the way evolution proceeds, as compared with the natural evolutionary mechanisms that have operated for the past 4 billion years.

Plants are more readily manipulated by genetic engineering than are animals because

(A) plant genes do not contain introns.

(B) more vectors are available for transferring recombinant

DNA into plant cells.

(C) a somatic plant cell can often give rise to a complete

plant.

(D) plant cells have larger nuclei.

A paleontologist has recovered a bit of tissue from the 400-year old preserved skin of an extinct dodo (a bird). To compare a specific region of the DNA from a sample with DNA from living birds, which of the following would be most helpful in increasing the amount of dodo DNA available for testing?

(A) SNP analysis

(B) polymerase chain reaction (PCR)

(C) electroporation

(D) gel electrophoresis

The water in the Yellowstone National Park hot springs shown here is around 160掳F (70掳C). Biologists assumed that no species of organisms couldlive in water above about 130掳F (55掳C), so they were surprised to find several species of bacteria there, now called thermophiles(鈥渉eat-lovers鈥). You鈥檝e learned in this chapter how an enzyme from one species, Thermus aquaticus, made feasible one of the most important DNA-based techniques used in labs today.Identify the enzyme, and indicate the value of its being isolated from a thermophile. Suggest other reasons why enzymes from this bacterium (or other thermophiles) might also be valuable.

Looking at Figure 20.15, what does it mean for a SNP to be 鈥渓inked鈥 to a disease-associated allele? How does this allow the SNP to be used as a genetic marker?(See Concept 15.3.)

See all solutions

Recommended explanations on Biology Textbooks

View all explanations

What do you think about this solution?

We value your feedback to improve our textbook solutions.

Study anywhere. Anytime. Across all devices.