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Which of the following tools of DNA technology is incorrectly paired with its use?

(A) electrophoresis鈥攕eparation of DNA fragments

(B) DNA ligase鈥攃utting DNA, creating sticky ends of restriction

fragments

(C) DNA polymerase鈥攑olymerase chain reaction to amplify

sections of DNA

(D) reverse transcriptase鈥攑roduction of cDNA from

mRNA

Short Answer

Expert verified

(A) The option 鈥渆lectrophoresis鈥攕eparation of DNA fragments鈥 is false.

(B) The option 鈥淒NA ligase鈥攃utting DNA, creating sticky ends of restriction fragments鈥 is true.

(C) The option 鈥淒NA polymerase鈥攑olymerase chain reaction to amplify

sections of DNA鈥 is false.

(D)The option 鈥渞everse transcriptase鈥攑roduction of cDNA from mRNA鈥 is false.

Step by step solution

01

Step 1: Tools of DNA technology

DNA technology is the molecular technique that involves finding the desired DNA fragments from the genome of an organism, inserting them in a suitable vector, and forming recombinant DNA. After this, it is multiplied in a host.

The task of DNA technology is not accomplished without using some tools that are mandatory to use. These tools constitute a vector, restriction enzymes, other enzymes like DNA ligase, DNA polymerase, reverse transcriptase, a host organism, and gel electrophoresis.

02

Explanation of option ‘(A)’

Electrophoresis is a technique that involves the use of a gel matrix to separate DNA and proteins on the basis of the size of molecules and the electric charge present on them.

Thus, electrophoresis and separation of DNA fragments are correctly paired.

Therefore, the given option is false.

03

Explanation of option ‘(B)’

DNA ligase is an enzyme that catalyzes the synthesis reaction that involves the formation of a phosphodiester bond between two DNA strands and joining them. DNA molecules are cut and digested by using restriction enzymes that also create sticky ends in DNA fragments.

Thus, cutting DNA and creatingsticky ends in DNA fragments do not involve DNA ligases.

Therefore, the given option is true.

04

Explanation of option ‘(C)’

DNA polymerases are enzymes involved in genome replication and maintenance and are also used in DNA technology to amplify the desired DNA fragments. They are used for DNA cloning, polymerase chain reaction, and genome sequencing.

Thus, DNA polymerases are used to amplify a section of DNA in the polymerase chain reaction.

Therefore, the given statement is false.

05

Explanation of option ‘(D)’

Reverse transcriptase is the enzyme that catalyzes the reverse process of central dogma that is reverse transcription. It makes complementary DNA copies of an RNA molecule.

Thus, reverse transcriptase synthesizes DNA copies from mRNA that is called complementary DNA.

Therefore, the given statement is false.

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