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The article "Production of Gaseous Nitrogen in Human Steady-State Conditions" (J. of Applied Physiology, 1972: 155-159) reports the following observations on the amount of nitrogen expired (in liters) under four dietary regimens: (1) fasting, (2) \(23 \%\) protein, (3) \(32 \%\) protein, and (4) \(67 \%\) protein. Use the Kruskal-Wallis test at level .05 to test equality of the corresponding \(\mu_{i}\) 's. $$ \begin{array}{llllll} \text { 1. } & 4.079 & 4.859 & 3.540 & 5.047 & 3.298 \\ \text { 2. } & 4.368 & 5.668 & 3.752 & 5.848 & 3.802 \\ \text { 3. } & 4.169 & 5.709 & 4.416 & 5.666 & 4.123 \\ \text { 4. } & 4.928 & 5.608 & 4.940 & 5.291 & 4.674 \\ \text { 1. } & 4.679 & 2.870 & 4.648 & 3.847 & \\ \text { 2. } & 4.844 & 3.578 & 5.393 & 4.374 & \\ 3 . & 5.059 & 4.403 & 4.496 & 4.688 & \\ 4 . & 5.038 & 4.905 & 5.208 & 4.806 & \end{array} $$

Short Answer

Expert verified
Perform the Kruskal-Wallis test to compare the four regimens.

Step by step solution

01

Arrange Data and Calculate Ranks

First, we need to list all the observations from the four dietary regimens: 1. Fasting: 4.079, 4.859, 3.540, 5.047, 3.298, 4.679, 2.870, 4.648, 3.847 2. 23% protein: 4.368, 5.668, 3.752, 5.848, 3.802, 4.844, 3.578, 5.393, 4.374 3. 32% protein: 4.169, 5.709, 4.416, 5.666, 4.123, 5.059, 4.403, 4.496, 4.688 4. 67% protein: 4.928, 5.608, 4.940, 5.291, 4.674, 5.038, 4.905, 5.208, 4.806 Combine all observations, then rank them from smallest to largest, giving tied values their average rank. Keep track of which rank belongs to which group.
02

Compute Rank Sums

After ranking all observations, calculate the sum of the ranks for each dietary regimen. Denote these sums as \( R_1, R_2, R_3, \) and \( R_4 \) corresponding to the regimens 1 through 4, respectively.
03

Calculate the Kruskal-Wallis Statistic

The Kruskal-Wallis Statistic \( H \) is calculated using the formula: \[ H = \frac{12}{N(N+1)} \left( \frac{R_1^2}{n_1} + \frac{R_2^2}{n_2} + \frac{R_3^2}{n_3} + \frac{R_4^2}{n_4} \right) - 3(N+1) \]where \( N \) is the total number of observations, and \( n_i \) is the number of observations in group \( i \). Fill in the sums of ranks and the number of observations to calculate \( H \).
04

Determine Critical Value and Conclusion

For a Kruskal-Wallis test at \( \alpha = 0.05 \) with \( k-1 = 3 \) degrees of freedom (where \( k \) is the number of groups), find the critical value from the chi-squared distribution table. If the calculated \( H \) is greater than the critical value, reject the null hypothesis of equal means across groups. Otherwise, do not reject the null hypothesis.

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Key Concepts

These are the key concepts you need to understand to accurately answer the question.

Non-parametric Statistics
Non-parametric statistics play a crucial role when data do not meet the assumptions necessary for parametric tests. Unlike parametric tests, these types of statistics do not require our data to fit a normal distribution, making them versatile across various types of data.

They are especially useful when dealing with ordinal data or data that involves ranks instead of precise measurements. Non-parametric methods, like the Kruskal-Wallis test, allow us to conduct hypothesis testing without relying on parameters like the mean and variance, which are essential in parametric tests.

In certain situations, data may have outliers or be skewed, violating standard assumptions. Non-parametric methods provide a more robust approach under these circumstances. The flexibility of non-parametric statistics is one of their strongest advantages, as they can be used in a broad range of scenarios.
Hypothesis Testing
Hypothesis testing is a statistical method used to determine if there is enough evidence in a sample to conclude that a certain condition holds true for the entire population. It typically involves formulating two hypotheses: the null hypothesis ( H鈧 ) and the alternative hypothesis ( H鈧 ). The null hypothesis suggests that there is no effect or difference, while the alternative posits the opposite.

Decision making in hypothesis testing usually involves determining the likelihood of observing data at least as extreme as the sample observation, under the assumption that the null hypothesis is true. A significant result (usually determined by a p a value less than 0.05) suggests rejecting the null hypothesis in favor of the alternative.

The Kruskal-Wallis test leverages hypothesis testing to evaluate whether there are statistically significant differences between the distributions of multiple independent groups. It's used as a non-parametric alternative to the ANOVA when the data doesn't meet the assumptions of normality.
Rank Sum Test
The rank sum test is an essential part of non-parametric statistical methods. It involves ranking all observations from all groups together, irrespective of the group to which they belong. Once ranked, the sum of ranks for each specific group is calculated. These ranked sums are then used in further analysis.

This method is particularly useful when dealing with data that cannot be assumed to follow a specific distribution, common in non-parametric statistics. In the Kruskal-Wallis test, the rank sums of the groups are compared using a specific formula to determine statistical differences between group medians.

Using ranks rather than raw data helps to mitigate the influence of outliers or extreme values, which can skew results if actual measurements were used. The rank sum approach provides a more reliable measure of central tendency when using non-parametric statistical tests.
Chi-squared Distribution
In the Kruskal-Wallis test, the chi-squared distribution plays a critical role. The test statistic calculated during the test follows a chi-squared distribution with k-1 degrees of freedom, where k is the number of groups.

The chi-squared distribution is a probability distribution that is often used in hypothesis testing, primarily for goodness-of-fit tests and for tests of independence in contingency tables. It is valuable in determining if observed data deviate from what we would expect under the null hypothesis.

For the Kruskal-Wallis test, the calculated test statistic is compared against critical values derived from the chi-squared distribution. If the test statistic is larger than the critical value, it implies that there is enough statistical evidence to reject the null hypothesis, indicating significant differences among the group medians. This makes the chi-squared distribution an integral part of understanding whether the results of the Kruskal-Wallis test are statistically significant.

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Most popular questions from this chapter

The sign test is a very simple procedure for testing hypotheses about a population median assuming only that the underlying distribution is continuous. To illustrate, consider the following sample of 20 observations on component lifetime (hr): $$ \begin{array}{rrrrrrr} 1.7 & 3.3 & 5.1 & 6.9 & 12.6 & 14.4 & 16.4 \\ 24.6 & 26.0 & 26.5 & 32.1 & 37.4 & 40.1 & 40.5 \\ 41.5 & 72.4 & 80.1 & 86.4 & 87.5 & 100.2 & \end{array} $$ We wish to test \(H_{0}: \tilde{\mu}=25.0\) versus \(H_{\mathrm{a}}: \tilde{\mu}>25.0\). The test statistic is \(Y=\) the number of observations that exceed \(25 .\) a. Consider rejecting \(H_{0}\) if \(Y \geq 15\). What is the value of \(\alpha\) (the probability of a type I error) for this test? [Hint: Think of a "success" as a lifetime that exceeds 25.0. Then \(Y\) is the number of successes in the sample.] What kind of a distribution does \(Y\) have when \(\tilde{\mu}=25.0\) ? b. What rejection region of the form \(Y \geq c\) specifies a test with a significance level as close to \(.05\) as possible? Use this region to carry out the test for the given data.

The accompanying data resulted from an experiment to compare the effects of vitamin \(C\) in orange juice and in synthetic ascorbic acid on the length of odontoblasts in guinea pigs over a 6-week period ("The Growth of the Odontoblasts of the Incisor Tooth as a Criterion of the Vitamin C Intake of the Guinea Pig," J. of Nutrition, 1947: 491-504). Use the Wilcoxon rank-sum test at level .01 to decide whether true average length differs for the two types of vitamin \(\mathrm{C}\) intake. Compute also an approximate \(P\)-value. $$ \begin{array}{lrrrrrr} \text { Orange Juice } & 8.2 & 9.4 & 9.6 & 9.7 & 10.0 & 14.5 \\ & 15.2 & 16.1 & 17.6 & 21.5 & & \\ \text { Ascorbic Acid } & 4.2 & 5.2 & 5.8 & 6.4 & 7.0 & 7.3 \\ & 10.1 & 11.2 & 11.3 & 11.5 & & \end{array} $$

In an experiment to compare the bond strength of two different adhesives, each adhesive was used in five bondings of two surfaces, and the force necessary to separate the surfaces was determined for each bonding. For adhesive 1 , the resulting values were \(229,286,245,299\), and 250 , whereas the adhesive 2 observations were \(213,179,163\), 247 , and 225 . Let \(\mu_{i}\) denote the true average bond strength of adhesive type \(i\). Use the Wilcoxon rank-sum test at level \(.05\) to test \(H_{0}: \mu_{1}=\mu_{2}\) versus \(H_{\mathrm{a}}: \mu_{1}>\mu_{2}\).

The article "Effects of a Rice-Rich Versus Potato-Rich Diet on Glucose, Lipoprotein, and Cholesterol Metabolism in Noninsulin-Dependent Diabetics" (Amer. J. of Clinical Nutr., 1984: 598-606) gives the accompanying data on cholesterolsynthesis rate for eight diabetic subjects. Subjects were fed a standardized diet with potato or rice as the major carbohydrate source. Participants received both diets for specified periods of time, with cholesterol-synthesis rate (mmol/day) measured at the end of each dietary period. The analysis presented in this article used a distribution-free test. Use such a test with significance level \(.05\) to determine whether the true mean cholesterol-synthesis rate differs significantly for the two sources of carbohydrates. $$ \begin{aligned} &\text { Cholesterol-Synthesis Rate }\\\ &\begin{array}{lcccccccc} \hline \text { Subject } & \mathbf{1} & \mathbf{2} & \mathbf{3} & \mathbf{4} & \mathbf{5} & \mathbf{6} & \mathbf{7} & \mathbf{8} \\ \hline \text { Potato } & 1.88 & 2.60 & 1.38 & 4.41 & 1.87 & 2.89 & 3.96 & 2.31 \\ \text { Rice } & 1.70 & 3.84 & 1.13 & 4.97 & .86 & 1.93 & 3.36 & 2.15 \\ \hline \end{array} \end{aligned} $$

The accompanying data on cortisol level was reported in the article "Cortisol, Cortisone, and 11-Deoxycortisol Levels in Human Umbilical and Maternal Plasma in Relation to the Onset of Labor" (J. of Obstetric Gynaecology of the British Commonwealth, 1974: 737-745). Experimental subjects were pregnant women whose babies were deliverec between 38 and 42 weeks gestation. Group 1 individuals elected to deliver by Caesarean section before labor onset group 2 delivered by emergency Caesarean during inducec labor, and group 3 individuals experienced spontaneous labor. Use the Kruskal-Wallis test at level \(.05\) to test for equality of the three population means. $$ \begin{array}{lrrrrrr} \text { Group 1 } & 262 & 307 & 211 & 323 & 454 & 339 \\ & 304 & 154 & 287 & 356 & & \\ \text { Group 2 } & 465 & 501 & 455 & 355 & 468 & 362 \\ \text { Group 3 } & 343 & 772 & 207 & 1048 & 838 & 687 \end{array} $$

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