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Chapter 23: Q25P (page 630)

Consider a chromatography column in whichVs=Vm/5 Find the retention factor if K = 3 and if K = 30 .

Short Answer

Expert verified

a) Retention factor when K=3 is 0.6

b) Retention factor when isK=30 is 6 .

Step by step solution

01

Given Information:

Vs=Vm/5K=3,K=30

02

To find the Retention factor when K = 3 :

VS/Vm=1/5

Retention factor is given by;

k=K脳VsVmk=3K=3脳15K=35=0.6

03

To find the Retention factor when K = 30 :

K=30k=K脳VsVmk=30脳15k=305k=6

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Most popular questions from this chapter

23-38. What is the optimal flow rate in Figure 23-17 for best separation of solutes?

(a) A chromatography column with a length of 10.3 cmand inner diameter of 4.61mmis packed with a stationary phase that occupies 61.0% of the volume. If the volume flow rate is 1.13mL, find the linear velocity in cm/min.(b) How long does it take for solvent (which is the same as unretained solute) to pass through the column? (c) Find the retention time for a solute with a retention factor of 10.

For the separation of A and B by column 2 in Problem 23-32:

(a) If broadening is mainly due to longitudinal diffusion, how should the flow rate be changed to improve the resolution?

(b) If broadening is mainly due to the finite equilibrium time, how should the flow rate be changed to improve the resolution?

(c) If broadening is mainly due to multiple flow paths, what effect will flow rate have on the resolution?

A chromatographic procedure separatesof unknown mixture on a column with a length of 40cmand a diameter of 0.85cm . (a) What size column would you use to separate 100mgof the same mixture? (b) If the flow is 0.22mL/minon the small column, what volume flow rate should be used on the large column? (c) The mobile phase occupies 35%of the column volume. Calculate the linear velocity for the small column and the large column.

An open tubular column has an inner diameter of 207渭尘and the thickness of the stationary phase on the inner wall is 0.50渭尘. Unretained solute passes through in 63s and a particular solute emerges in 433s . Find the partition coefficient for this solute and find the fraction of time spent in the stationary phase.
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